长春西汀注射液减轻脂多糖诱导的大鼠急性肺损伤

发布时间：2018-07-27 11:26

【摘要】：目的:观察长春西汀注射液对脂多糖(lipopolysaccharide,LPS)诱导大鼠急性肺损伤(acute lung injury,ALI)的作用,并研究初步的作用机制。方法:雄性Wistar大鼠50只,随机分为正常对照组(control)、模型组(ALI组)以及长春西汀低剂量组、中剂量组和高剂量组,每组10只。正常对照组股静脉注射0.9%氯化钠注射液(5 m L/kg);模型组股静脉注射LPS 10 mg/kg;长春西汀低、中和高剂量组股静脉注射LPS 10 mg/kg,30 min后分别腹腔注射长春西汀注射液0.2 mg/kg、0.7 mg/kg和1.2 mg/kg。伊红染色观察肺部组织病理学切片,TUNEL法检测肺组织的细胞凋亡,分光光度法检测并计算肺组织髓过氧化物酶(MPO)活性,Western blot法检测肺组织中NF-κB、ICAM-1、VCAM-1、Bax与Bcl-2的蛋白水平。结果:与模型组相比,采用长春西汀给药后,明显减轻急性肺损伤的肺组织结构损伤与炎性细胞浸润,降低肺组织凋亡的细胞数与MPO活性,下调细胞中NF-κB、ICAM-1、VCAM-1与Bax的蛋白表达水平,上调Bcl-2的蛋白表达水平。结论:长春西汀注射液对急性肺损伤大鼠的肺组织具有保护作用,可能与降低肺组织中MPO活性,以及调控NF-κB、ICAM-1、VCAM-1、Bax与Bcl-2蛋白表达水平有关。
[Abstract]:Aim: to observe the effect of vinpocetine injection on (acute lung injury-induced Ali in rats induced by lipopolysaccharide (LPS), and to study its mechanism. Methods: fifty male Wistar rats were randomly divided into normal control (control), model group (ALI group), vinpocetine low dose group, middle dose group and high dose group with 10 rats in each group. In normal control group, 0.9% sodium chloride injection (5 m L/kg) was injected into femoral vein, LPS was injected 10 mg / kg by femoral vein in model group, and vinpocetine injection 0.2 mg / kg ~ 0.7 mg/kg and 1.2 mg 路kg ~ (-1) kg 路kg ~ (-1) LPS were injected intraperitoneally in low, medium and high dose groups after 30 min. Apoptosis of lung tissue was detected by Tunel method, and the activity of myeloperoxidase (MPO) in lung tissue was detected by spectrophotometry. The protein levels of NF- 魏 B, ICAM-1, VCAM-1, Bax and Bcl-2 in lung tissue were detected by Western blot. Results: compared with the model group, the administration of vinpocetine significantly alleviated the lung structure injury and inflammatory cell infiltration, decreased the number of apoptotic cells and MPO activity, and down-regulated the protein expression of NF- 魏 BnICAM-1VCAM-1 and Bax in acute lung injury. The protein expression level of Bcl-2 was up-regulated. Conclusion: the protective effect of vinpocetine injection on the lung tissue of rats with acute lung injury may be related to the decrease of MPO activity in lung tissue and the regulation of the expression of Bcl-2 protein.
【作者单位】：广东药科大学附属第一医院内二科;
【基金】：广东省财政技术研究开发与推广应用项目(No.2060403)
【分类号】：R563.8

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