红景天对哮喘小鼠气道炎症和气道重塑的影响
发布时间：2018-07-31 06:04
【摘要】：目的:探讨红景天对哮喘小鼠气道炎症和气道重塑的调控作用及机制。方法:40只C57bL/6小鼠随机分成对照组、哮喘组、布地奈德组、红景天低、高剂量组(0.21,1 g/kg),每组8只。小鼠分别于第0、14天以OVA致敏,第24天开始每天雾化吸入2.5%OVA激发并持续18 d,建立哮喘模型。红景天组致敏后每次激发前0.5 h予以红景天灌胃。刘氏染色法分类计数支气管肺泡灌洗液(BALF)中细胞数,肺组织病理学检查观察呼吸道炎症、胶原染色面积,ELISA法检测BALF中TGF-β1、IL-4和IFN-γ的浓度,免疫组化法检测肺组织中TGF-β1、IL-4和IFN-γ的表达。结果:与对照组相比较,哮喘组小鼠呼吸道炎症、胶原染色面积明显加重,肺组织中IL-4、IL-4/IFN-γ、TGF-β1升高,IFN-γ降低(P0.05);布地奈德和红景天能减轻小鼠气道炎症及胶原染色面积,降低肺组织中IL-4、IL-4/IFN-γ、TGF-β1,升高IFN-γ(P0.05)。结论:红景天能够抑制哮喘小鼠气道炎症和气道重塑,其机制可能是通过调节IL-4/IFN-γ失衡,抑制TGF-β1的表达有关。
[Abstract]:Objective: to investigate the effect and mechanism of Rhodiola on airway inflammation and airway remodeling in asthmatic mice. Methods 40 C57bL/6 mice were randomly divided into control group, asthma group, budesonide group, low salidroid group and high dose group (0.21 g/kg) with 8 mice in each group. The mice were sensitized with OVA on day 0 and day 14, respectively, and the asthma model was established by daily inhalation of 2.5%OVA for 18 days from day 24. Rhodiola group was sensitized 0.5 h before each stimulation. The number of cells in bronchoalveolar lavage fluid (BALF) was counted by Liu's staining method, the respiratory tract inflammation was observed by pathological examination of lung tissue, the concentration of TGF- 尾 _ (1) IL-4 and IFN- 纬 in BALF was detected by Elisa and the expression of TGF- 尾 _ (1) IL-4 and IFN- 纬 in lung tissue was detected by immunohistochemistry. Results: compared with the control group, the airway inflammation and collagen staining area in asthmatic mice were significantly increased, IL-4 / IFN- 尾 1 increased and IFN- 尾 1 decreased (P0.05), budesonide and Rhodiola could reduce airway inflammation and collagen staining area in mice. Decrease IL-4 / IFN- 纬 TGF- 尾 1 and increase IFN- 纬 in lung tissue (P0.05). Conclusion: Rhodiola can inhibit airway inflammation and airway remodeling in asthmatic mice by regulating IL-4 / IFN- 纬 imbalance and inhibiting the expression of TGF- 尾 _ 1.
【作者单位】： 泸州医学院附属医院急诊科;泸州医学院附属医院呼吸一科;
【分类号】：R562.25
，
本文编号：2154659
[Abstract]:Objective: to investigate the effect and mechanism of Rhodiola on airway inflammation and airway remodeling in asthmatic mice. Methods 40 C57bL/6 mice were randomly divided into control group, asthma group, budesonide group, low salidroid group and high dose group (0.21 g/kg) with 8 mice in each group. The mice were sensitized with OVA on day 0 and day 14, respectively, and the asthma model was established by daily inhalation of 2.5%OVA for 18 days from day 24. Rhodiola group was sensitized 0.5 h before each stimulation. The number of cells in bronchoalveolar lavage fluid (BALF) was counted by Liu's staining method, the respiratory tract inflammation was observed by pathological examination of lung tissue, the concentration of TGF- 尾 _ (1) IL-4 and IFN- 纬 in BALF was detected by Elisa and the expression of TGF- 尾 _ (1) IL-4 and IFN- 纬 in lung tissue was detected by immunohistochemistry. Results: compared with the control group, the airway inflammation and collagen staining area in asthmatic mice were significantly increased, IL-4 / IFN- 尾 1 increased and IFN- 尾 1 decreased (P0.05), budesonide and Rhodiola could reduce airway inflammation and collagen staining area in mice. Decrease IL-4 / IFN- 纬 TGF- 尾 1 and increase IFN- 纬 in lung tissue (P0.05). Conclusion: Rhodiola can inhibit airway inflammation and airway remodeling in asthmatic mice by regulating IL-4 / IFN- 纬 imbalance and inhibiting the expression of TGF- 尾 _ 1.
【作者单位】： 泸州医学院附属医院急诊科;泸州医学院附属医院呼吸一科;
【分类号】：R562.25
，
本文编号：2154659
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