慢性阻塞性肺疾病患者呼出气冷凝液IL-17、IL-10、8-iso-PG的测定及临床意义

发布时间：2018-08-19 12:15

【摘要】：目的探讨慢性阻塞性肺疾病(简称慢阻肺)急性加重期患者呼出气冷凝液(EBC)中细胞因子水平的变化及临床意义。方法前瞻性收集2015年3月至2016年8月在深圳市第七人民医院呼吸内科住院的慢阻肺急性加重患者30例和21例健康对照志愿者。采集各组一般资料和EBC,采用酶联免疫法测定EBC中白细胞介素-17(IL-17)、IL-10、8-异前列腺素(8-iso-PG)水平,并同时进行肺功能检测。结果慢阻肺急性加重组患者治疗前后EBC中IL-17、8-iso-PG水平均较对照组升高[(10.74±1.02)ng/L、(5.65±0.88)ng/L vs.(3.36±0.61)ng/L,(12.35±2.25)ng/L、(9.65±1.22)ng/L vs.(6.93±1.15)ng/L,P0.05],而IL-10水平则均较对照组低[(1.68±0.17)ng/L、(2.59±0.31)ng/L vs.(2.85±0.43)ng/L,P0.05]。慢阻肺急性加重组患者治疗后IL-17、8-iso-PG水平较治疗前显著下降[(5.65±0.88)ng/L vs.(10.74±1.02)ng/L,P0.05;(9.65±1.22)ng/L vs.(12.35±2.25)ng/L,P0.05],而IL-10水平较治疗前升高[(2.59±0.31)ng/L vs.(1.68±0.17)ng/L,P0.05]。慢阻肺急性加重组患者治疗后肺功能指标FEV1、FVC、FEV1%pred较治疗前显著增加(P0.01)。慢阻肺急性加重患者治疗前后IL-17、IL-10、8-iso-PG与FEV1%pred均无显著相关性(P0.05)。结论慢阻肺急性加重患者EBC的IL-17、IL-10、8-iso-PG可作为监测慢阻肺急性加重患者治疗期间气道炎症及氧化应激水平的重要指标。
[Abstract]:Objective to investigate the changes and clinical significance of cytokines in (EBC) of patients with acute exacerbation of chronic obstructive pulmonary disease (COPD). Methods from March 2015 to August 2016, 30 patients with acute exacerbation of COPD and 21 healthy volunteers were enrolled in Department of Respiratory Medicine, Seventh people's Hospital of Shenzhen City. The general data of each group and EBC were collected, and the levels of interleukin-17 (IL-17) IL-108-isoprostaglandin (8-iso-PG) in EBC were determined by enzyme-linked immunosorbent assay (Elisa). Results before and after treatment, the levels of IL-17N 8-iso-PG in patients with acute exacerbation of COPD were significantly higher than those in the control group [(10.74 卤1.02) ng / L, (5.65 卤0.88) ng/L vs. (3.36 卤0.61) ng / L, (12.35 卤2.25) ng / L, (9.65 卤1.22) ng/L vs. (6.93 卤1.15) ngL / L], while the IL-10 levels were lower than those in the control group [(1.68 卤0.17) ngL / L, (2.59 卤0.31) ng/L vs (2.85 卤0.43) ng / L P 0.05]. The levels of IL-17N 8-iso-PG in patients with acute exacerbation of COPD after treatment were significantly lower than those before treatment [(5.65 卤0.88) ng/L vs. (10.74 卤1.02) ng / L, P 0.05; (9.65 卤1.22) ng/L vs (12.35 卤2.25) ng / L, P 0.05], while IL-10 levels were higher than those before treatment [(2.59 卤0.31) ng/L vs. (1.68 卤0.17) ng / L, P 0.05]. After treatment, the pulmonary function index FEV1 FVCFVCFEV1 pred in patients with acute exacerbation of COPD was significantly higher than that before treatment (P0.01). There was no significant correlation between IL-17 and IL-108-iso-PG and FEV1%pred before and after treatment in patients with acute exacerbation of COPD (P0.05). Conclusion IL-17 IL-10 8-iso-PG of EBC in patients with acute exacerbation of COPD can be used as an important index to monitor airway inflammation and oxidative stress during treatment in patients with acute exacerbation of COPD.
【作者单位】：深圳市第七人民医院呼吸内科;广州医科大学附属一院呼吸内科广州呼吸疾病研究所;
【基金】：深圳市科技计划项目(JCYJ20150402092102039)
【分类号】：R563.9

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