雾化吸入重组人角质细胞生长因子-2对烟雾吸入性损伤兔氧合作用及炎症反应的影响
发布时间：2018-08-29 16:27
【摘要】：目的:观察雾化吸入重组人角质细胞生长因子-2对烟雾吸入性损伤兔氧合作用及炎症反应的影响。方法:将120只新西兰大耳白兔制成烟雾吸入性损伤模型,随机分为五组,每组各24只。一组动物致伤后正常饮食饮水,留作空白对照组;其余各组在烟雾吸入性损伤模型达成后开始,每天分别予以雾化吸入5ml PBS及5ml PBS外加1mg/kg、2mg/kg、5mg/kg的rhKGF-2;于实验组雾化治疗1d、3d、5d、7d后,各组各取6只动物麻醉后行耳中动脉血气分析,心脏采血检测炎症指标IL-6、IL-8、IL-10及MCP-1,PBS缓冲液灌洗左肺并检测BALF中炎症指标IL-6、IL-8、IL-10及MCP-1水平,取右下肺下半叶行组织病理学检查。结果:1、整体分析PaO_2、SaO_2发现,药物因素有显著作用(P0.001),PaO_2、SaO_2组内多重比较发现1mg组、2mg组及5mg组分别与PBS组相比显示均有显著差异(P0.05),且PaO_2组内5mg vs 1mg有显著差异。PH、PaO_2、PaCO_2、SaO_2及HCO_3~-各组整体分析发现时间因素有显著作用,PH、PaO_2、PaCO_2、SaO_2各组P0.001,HCO_3~-指标P0.05;PH、PaO_2、SaO_2各指标组内两两比较发现3d vs 1d、5d vs 1d及7d vs 1d有显著差异(P0.05),PH指标组内比较7d vs 3d有显著差异(P0.05),PaCO_2组内多重比较发现3d vs 1d、5d vs 1d有显著差异(P0.05),HCO_3~-组内多重比较发现5d vs 1d有显著差异(P0.05)。2、各组动物病理检查见肺泡腔内均有程度不等的炎性渗出及出血表现,部分肺泡腔囊状扩张或萎陷,细支气管粘膜出现不同程度的变性、坏死,腔内炎性渗出物附着。且随着时间的变化各组肺泡腔内及间质内中性粒细胞浸润、渗出液及出血等情况,均逐渐减轻。且见治疗5d、7d后上述变化以5mg组最显著。3、BALF中的炎症指标IL-6、IL-8及IL-10的整体分析结果显示药物及时间均有显著作用(P0.05)。药物因素上,IL-6组内多重比较发现,2mg组vs PBS组、5mg组vs PBS组均有差异(P0.05);IL-8组内多重比较,1mg组vs PBS组、2mg组vs PBS组、5mg组vs PBS组均有差异(P0.05);IL-10组内多重比较,2mg组vs 1mg组有差异(P0.05)。时间因素上,IL-6指标组内多重比较发现7d vs 1d有统计学意义(P0.01),IL-8组内多重比较发现5d vs 1d、7d vs 1d、7d vs 3d均有显著差异(P0.05),IL-10组内多重比较发现3d vs 1d、7d vs 3d有显著差异(P0.05);BALF中MCP-1的检测结果无统计学意义(P0.05)。4、血清中的炎症指标检测结果未发现药物有显著影响,近一半的血清样品中未测到IL-6。血清中IL-8、IL-10的整体分析均发现时间因素有显著作用(P0.05)。血清IL-8组内多重比较发现5d vs 1d、7d vs 1d有显著差异(P0.01),7d vs 3d有显著差异(P0.05);血清IL-10组内多重比较发现7d vs 1d有显著作用(P0.01)。血清中MCP-1的检测结果无统计学意义(P0.05)。结论:雾化吸入rhKGF-2治疗烟雾吸入性损伤能显著改善机体氧合作用,减少肺部炎症反应,但对全身炎症反应无明显影响。
[Abstract]:Aim: to observe the effect of nebulized inhalation of recombinant human keratinocyte growth factor-2 on oxygenation and inflammatory response in rabbits with smoke inhalation injury. Methods: 120 New Zealand white rabbits were randomly divided into five groups with 24 rabbits in each group. One group of animals had normal diet and drinking water after injury, and the other groups were given atomized inhalation of 5ml PBS and 5ml PBS respectively every day after the model of smoke inhalation injury was achieved, and the other groups were treated with rhKGF-2; of 1 mg / kg 2 mg / kg + 5 mg / kg rhKGF-2; for 7 days after 1 day of atomization treatment. After anesthesia, the blood gas of middle ear artery was analyzed in 6 animals in each group. IL-6,IL-8,IL-10 and MCP-1,PBS buffer were used to lavage the left lung, and the levels of IL-6,IL-8,IL-10 and MCP-1 in BALF were detected. The lower lobe of right lower lung was taken for histopathological examination. Result: 1, overall analysis of PaO_2,SaO_2 found, Drug factors had significant effect (P0.001) in comparison of the two groups of Pao _ 2C _ 2 and Sao _ P _ 2. Significant differences were found between the 1mg group (2mg) and the 5mg group (P0.05), and the 5mg vs 1mg in the PaO_2 group was significantly different (P < 0.05). The overall analysis of Phapao _ 2 / Paco _ 2C _ 2O _ 2 and HCO_3~- found that there were significant time factors in the whole analysis of each group. P0.001 HCO3HCO3 Index P0.05P0.05 PHPAO2SaO2The results showed that there were significant differences between the three groups (P0.05) in 3 d vs 1 d 5 d vs 1 d and 7 d vs 1 d (P0.05) in PH group there was significant difference between 7 d vs 3D group and 3 d vs 1d group. (P05) significant difference was found in 3 d vs 1d 5 d vs 1d. (P05) significant difference was found in 3 d vs 1d 5 d vs 1d. (P0.05) significant difference was found in 3 d vs 1d 5 d vs 1d in PH index group. (P0.05) significant difference was found between the two groups. (P05) significant difference was found between the two groups. (P05) significant difference was found in 3 d vs 1d 5 d vs 1d in PH group (P0.05). Multiple comparisons within the group showed that there was a significant difference in vs 1d at 5 days (P0.05). Pathological examination showed that there were inflammatory exudation and hemorrhage in alveolar cavity in all groups. Part of alveolar cavity dilated or collapsed, bronchiolus mucosal degeneration, necrosis, intraluminal inflammatory exudate attachment. With the change of time, neutrophil infiltration, exudate and hemorrhage in alveolar cavity and interstitial were gradually alleviated. The results of IL-6,IL-8 and IL-10 in 5mg group showed that the drug and time had significant effect (P0.05). There was significant difference in vs PBS group (P05) in vs PBS 5mg group (P05). There was significant difference between 1mg vs PBS group, 2mg vs PBS group, 5mg group vs PBS group (P0.05) and 2mg group vs 1mg group (P0.05). Significant differences were found in multiple indexes of IL-6 in 7 day vs 1 day group and 7 d vs 1 d 7 d vs 3 d in IL-8 group (P0.05). There was significant difference in MCP-1 in vs 1 d 7 d vs 3 d in IL-10 group (P0.05). The results were not statistically significant (P0.05). 4. No significant effect was found on the inflammatory indexes in serum. IL-6. was not detected in nearly half of the serum samples The whole analysis of IL-8,IL-10 in serum showed that time factor had significant effect (P 0.05). Significant difference was found in serum IL-8 group (P0. 01) on 5 d vs 1 d, 7 d vs 1 d on 7 d vs 3 d (P0.05), and on 7 d vs 1 d in serum IL-10 group (P0. 01). The results of MCP-1 in serum were not statistically significant (P0.05). Conclusion: aerosol inhalation of rhKGF-2 can significantly improve the oxygenation and reduce the pulmonary inflammatory response, but has no obvious effect on systemic inflammatory reaction.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R56
[Abstract]:Aim: to observe the effect of nebulized inhalation of recombinant human keratinocyte growth factor-2 on oxygenation and inflammatory response in rabbits with smoke inhalation injury. Methods: 120 New Zealand white rabbits were randomly divided into five groups with 24 rabbits in each group. One group of animals had normal diet and drinking water after injury, and the other groups were given atomized inhalation of 5ml PBS and 5ml PBS respectively every day after the model of smoke inhalation injury was achieved, and the other groups were treated with rhKGF-2; of 1 mg / kg 2 mg / kg + 5 mg / kg rhKGF-2; for 7 days after 1 day of atomization treatment. After anesthesia, the blood gas of middle ear artery was analyzed in 6 animals in each group. IL-6,IL-8,IL-10 and MCP-1,PBS buffer were used to lavage the left lung, and the levels of IL-6,IL-8,IL-10 and MCP-1 in BALF were detected. The lower lobe of right lower lung was taken for histopathological examination. Result: 1, overall analysis of PaO_2,SaO_2 found, Drug factors had significant effect (P0.001) in comparison of the two groups of Pao _ 2C _ 2 and Sao _ P _ 2. Significant differences were found between the 1mg group (2mg) and the 5mg group (P0.05), and the 5mg vs 1mg in the PaO_2 group was significantly different (P < 0.05). The overall analysis of Phapao _ 2 / Paco _ 2C _ 2O _ 2 and HCO_3~- found that there were significant time factors in the whole analysis of each group. P0.001 HCO3HCO3 Index P0.05P0.05 PHPAO2SaO2The results showed that there were significant differences between the three groups (P0.05) in 3 d vs 1 d 5 d vs 1 d and 7 d vs 1 d (P0.05) in PH group there was significant difference between 7 d vs 3D group and 3 d vs 1d group. (P05) significant difference was found in 3 d vs 1d 5 d vs 1d. (P05) significant difference was found in 3 d vs 1d 5 d vs 1d. (P0.05) significant difference was found in 3 d vs 1d 5 d vs 1d in PH index group. (P0.05) significant difference was found between the two groups. (P05) significant difference was found between the two groups. (P05) significant difference was found in 3 d vs 1d 5 d vs 1d in PH group (P0.05). Multiple comparisons within the group showed that there was a significant difference in vs 1d at 5 days (P0.05). Pathological examination showed that there were inflammatory exudation and hemorrhage in alveolar cavity in all groups. Part of alveolar cavity dilated or collapsed, bronchiolus mucosal degeneration, necrosis, intraluminal inflammatory exudate attachment. With the change of time, neutrophil infiltration, exudate and hemorrhage in alveolar cavity and interstitial were gradually alleviated. The results of IL-6,IL-8 and IL-10 in 5mg group showed that the drug and time had significant effect (P0.05). There was significant difference in vs PBS group (P05) in vs PBS 5mg group (P05). There was significant difference between 1mg vs PBS group, 2mg vs PBS group, 5mg group vs PBS group (P0.05) and 2mg group vs 1mg group (P0.05). Significant differences were found in multiple indexes of IL-6 in 7 day vs 1 day group and 7 d vs 1 d 7 d vs 3 d in IL-8 group (P0.05). There was significant difference in MCP-1 in vs 1 d 7 d vs 3 d in IL-10 group (P0.05). The results were not statistically significant (P0.05). 4. No significant effect was found on the inflammatory indexes in serum. IL-6. was not detected in nearly half of the serum samples The whole analysis of IL-8,IL-10 in serum showed that time factor had significant effect (P 0.05). Significant difference was found in serum IL-8 group (P0. 01) on 5 d vs 1 d, 7 d vs 1 d on 7 d vs 3 d (P0.05), and on 7 d vs 1 d in serum IL-10 group (P0. 01). The results of MCP-1 in serum were not statistically significant (P0.05). Conclusion: aerosol inhalation of rhKGF-2 can significantly improve the oxygenation and reduce the pulmonary inflammatory response, but has no obvious effect on systemic inflammatory reaction.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R56
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