寒燥对慢性阻塞性肺疾病模型肺部蛋白酶-抗蛋白酶失衡的影响

发布时间：2018-09-12 20:10

【摘要】：目的:观察寒燥对慢性阻塞性肺疾病(COPD)模型肺部基质金属蛋白酶-2(MMP-2)m RNA、基质金属蛋白酶-9(MMP-9)m RNA、基质金属蛋白酶抑制剂-1(TIMP-1)m RNA在肺部表达的影响,为寒燥型COPD特点的揭示提供参考。方法:利用气管滴注弹性蛋白酶结合熏烟的方法建立COPD模型,气管滴注弹性蛋白酶、熏烟结合寒燥环境应激的方法建立寒燥型COPD模型,利用Real-time PCR法检测模型肺组织中MMP-2、MMP-9、TIMP-1的m RNA表达。结果:MMP-2 m RNA的表达量COPD寒燥组和COPD模型组均高于对照组(P0.01),COPD寒燥组高于COPD模型组(P0.01);MMP-9 m RNA的表达量COPD寒燥组和COPD模型组均高于对照组(P0.01),COPD寒燥组高于COPD模型组(P0.05);TIMP-1 m RNA的表达量COPD寒燥组和COPD模型组均高于对照组(P0.01);MMP-9 m RNA/TIMP-1 m RNA的比值,COPD寒燥组COPD模型组对照组。结论:寒燥可以增加COPD模型肺部MMP-2、MMP-9、TIMP-1的m RNA表达,MMP-9 m RNA/TIMP-1 m RNA比值增大,表明气道壁以炎性反应为主,导致组织破坏,形成肺气肿。
[Abstract]:Objective: to observe the effect of cold dryness on the expression of MMP-2) m RNA, matrix metalloproteinase-9 (TIMP-1) m RNA) in lung of (COPD) model of chronic obstructive pulmonary disease (COPD), and to provide reference for revealing the characteristics of COPD in cold dryness type. Methods: COPD model was established by trachea instillation of elastase combined with fumigation, and the COPD model of cold dryness was established by instillation of elastase and smoke combined with cold dryness environment stress. The m RNA expression of MMP-2,MMP-9,TIMP-1 in lung tissue was detected by Real-time PCR method. Results the expression of MMP-9 m RNA in COPD cold dryness group and COPD model group was higher than that in COPD model group (P0.01). The expression of MMP-9 m RNA in COPD cold dryness group and COPD model group were higher than that in control group (P0.01) and COPD model group (P0.05). The expression of MMP-9 m RNA/TIMP-1 m RNA in COPD cold dryness group and COPD model group was higher than that in control group (P0.01). The ratio of MMP-9 m RNA/TIMP-1 m RNA in COPD cold dryness group and COPD model group was higher than that in COPD model group. Conclusion: cold dryness can increase the expression of m RNA and the ratio of MMP-9 m RNA/TIMP-1 m RNA in lung MMP-2,MMP-9,TIMP-1 of COPD model, indicating that the airway wall is mainly inflammatory, leading to tissue destruction and emphysema.
【作者单位】：新疆医科大学附属中医医院国家中医临床研究基地研究部;
【基金】：新疆维吾尔自治区青年科技创新人才培养工程(No.2014731016)~~
【分类号】：R563.9;R563.8

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