慢性阻塞性肺疾病患者血清细胞凋亡因子、白三烯B4水平及临床意义
发布时间：2018-09-17 09:06
【摘要】：【目的】 探讨慢性阻塞性肺疾病(COPD)患者急性发作期及缓解期以及正常对照组血清细胞凋亡因子、白三烯B4水平及临床意义。 【方法】 采集COPD急性加重期、稳定期患者和健康者外周血血清，采用双抗体夹心ELISA法进行测定人血清细胞凋亡因子（Fas/APO-1），固相夹心法酶联免疫吸附实验（ELISA）测定人白三烯B4（LTB4）。 【结果】 急性发作期患者的血清Fas/APO-1、LTB4明显升高、经临床治疗缓解后,患者血清Fas/APO-1、LTB4均显著降低(P0.05),但仍明显高于对照组(P0.01)。 【结论】 COPD患者从急性发作期至缓解期,血清Fas/APO-1、LTB4均发生了显著变化，均参与了COPD气道炎症反应，并与炎性损伤程度有关，因此COPD患者急性发作期Fas/APO-1和LTB4监测对于病情判断、指导临床治疗具有一定意义。
[Abstract]:[objective] to investigate the serum apoptotic factors in patients with chronic obstructive pulmonary disease (COPD) during acute attack, remission and normal control. The level of leukotriene B4 and its clinical significance. [methods] Peripheral blood serum was collected from patients with acute exacerbation of COPD, stable patients and healthy persons. Human serum apoptotic factor (Fas/APO-1) was determined by double antibody sandwich ELISA method, and human leukotriene B4 (LTB4) was detected by (ELISA) with solid phase sandwich assay. [results] the serum Fas/APO-1,LTB4 of patients with acute attack was significantly increased. After clinical treatment, the serum Fas/APO-1,LTB4 of patients decreased significantly (P0.05), but still significantly higher than that of the control group (P0.01). [conclusion] Serum Fas/APO-1,LTB4 in patients with COPD changed significantly from acute attack to remission. All of them were involved in the airway inflammation of COPD, and were related to the degree of inflammatory injury. Therefore, the monitoring of Fas/APO-1 and LTB4 in patients with COPD during acute attack is of significance in judging the condition and guiding the clinical treatment.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R563.9
本文编号：2245385
[Abstract]:[objective] to investigate the serum apoptotic factors in patients with chronic obstructive pulmonary disease (COPD) during acute attack, remission and normal control. The level of leukotriene B4 and its clinical significance. [methods] Peripheral blood serum was collected from patients with acute exacerbation of COPD, stable patients and healthy persons. Human serum apoptotic factor (Fas/APO-1) was determined by double antibody sandwich ELISA method, and human leukotriene B4 (LTB4) was detected by (ELISA) with solid phase sandwich assay. [results] the serum Fas/APO-1,LTB4 of patients with acute attack was significantly increased. After clinical treatment, the serum Fas/APO-1,LTB4 of patients decreased significantly (P0.05), but still significantly higher than that of the control group (P0.01). [conclusion] Serum Fas/APO-1,LTB4 in patients with COPD changed significantly from acute attack to remission. All of them were involved in the airway inflammation of COPD, and were related to the degree of inflammatory injury. Therefore, the monitoring of Fas/APO-1 and LTB4 in patients with COPD during acute attack is of significance in judging the condition and guiding the clinical treatment.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R563.9
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