过敏性哮喘对小鼠动脉粥样硬化斑块发生发展及稳定性的影响

发布时间：2018-10-08 10:04

【摘要】：目的观察过敏性哮喘对apoE-/-小鼠动脉粥样硬化(AS)病变发生及发展不同时期的影响。方法取6周龄的apoE-/-小鼠,以卵清蛋白(OVA)致敏后给予雾化吸入,激发哮喘发作以建立过敏性哮喘模型,激发2周后检测小鼠肺部病理改变判断造模是否成功。造模成功后分别于激发2周、4周、8周及16周时处死小鼠,取主动脉根部进行冰冻切片,油红O染色并计算AS斑块相对面积,CD68荧光染色检测斑块内巨噬细胞含量,天狼星红染色检测斑块内胶原含量。结果与对照组相比,过敏性哮喘apoE-/-小鼠主动脉根部在激发2周时就有明显的斑块形成和巨噬细胞浸润,并且随着激发时间的延长,斑块面积和斑块内巨噬细胞含量始终高于对照组,同时斑块内胶原含量下降。结论过敏性哮喘促进apoE-/-小鼠AS斑块的形成和发展,并导致其稳定性降低。
[Abstract]:Objective to observe the effect of allergic asthma on the occurrence and development of atherosclerotic (AS) in apoE-/- mice. Methods six week-old apoE-/- mice were sensitized with ovalbumin (OVA) and inhaled by atomization to induce asthma to establish allergic asthma model. After 2 weeks of stimulation, the lung pathological changes of mice were detected to determine whether the model was successful or not. The mice were killed at 2 weeks, 4 weeks, 8 weeks and 16 weeks after successful modeling. The aortic roots were taken for frozen sections. The relative area of AS plaques was stained and CD68 fluorescence staining was used to detect the content of macrophages in the plaques. Collagen content in plaque was detected by Sirius red staining. Results compared with the control group, the aortic root of apoE-/- mice with allergic asthma had obvious plaque formation and macrophage infiltration during 2 weeks of stimulation, and with the prolongation of the stimulation time, the aortic root of allergic asthma mice had significant plaque formation and macrophage infiltration during 2 weeks of stimulation. Plaque area and macrophage content in plaque were always higher than those in control group, while collagen content in plaque decreased. Conclusion allergic asthma can promote the formation and development of AS plaque in apoE-/- mice and decrease its stability.
【作者单位】：西安交通大学医学部第一附属医院心内科;延安大学附属医院神经内科;
【基金】：国家自然科学基金杰出青年基金资助项目(No.81025002) 国家重点基础研究发展计划(“973计划”,No.2012CB517804)~~
【分类号】：R562.25

【相似文献】