血清中TGF-β1、MDA、SOD和IL-12B、IL-6、IL-1β基因多态性与宁夏煤工尘肺的关系研究

发布时间：2018-10-09 13:21

【摘要】：目的研究白细胞介素-12B（1188A/C位点）、白细胞介素-6(634C/G位点)、白细胞介素-1β(511C/T位点)基因多态性与宁夏汉族煤工尘肺(CWP)的关系；测定尘肺患者血清中转化生长因子-β1（TGF-β1）、丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性水平，以探讨其在煤工尘肺发病中的作用和意义。方法采用聚合酶链反应-限制片段长度多态性（PCR-RFLP）法对120例煤工尘肺患者和120例接尘对照人群进行IL-12B基因1188A/C位点、IL-6基因634C/G位点和IL-1β基因511C/T位点多态性分型分析，并随机抽取30例煤工尘肺患者和40例正常健康人群分别应用硫代巴比妥酸(TBA)比色法、黄嘌呤氧化酶法和酶联免疫吸附法（EL ISA）进行MDA含量、SOD活性和TGF-β1含量的测定。结果基因IL-12B、IL-6、IL-1β多态位点的基因型在两组对象中的分布符合Hardy一weinberg平衡，，工龄、工种分布和烟龄在煤工尘肺组与接尘对照组之间比较无统计学差异（均P＞0.05）。IL-12B基因1188位点在煤工尘肺组和接尘对照组中检出三种基因型AA、AC、CC型，这三种基因型在煤工尘肺组和接尘对照组间分布无显著性差异（P＞0.05）；等位基因C在煤工尘肺组和接尘对照组中所占比例分别为45.8％和36.7％，差异有统计学意义（P＜0.05）；IL-1β (C-511T)位点的三种基因型在煤工尘肺组和接尘对照组间分布差异无统计学意义（P＞0.05）；等位基因在煤工尘肺组和和接尘对照组比较无统计学意义（P＞0.05）；煤工尘肺组IL-6基因634位点基因型CC和CG+GG分布频率(66.7%、33.3%)和接尘对照组（52.5%、47.5%）比较差异有统计学意义（P㩳0.05）；等位基因在两组分布差异无统计学意义（P＞0.05）。煤工尘肺患者血清中MDA、TGF-β1含量分别为(41.61士33.35)nmol/ml、(27.76士13.86) ng/ml，明显高于健康人群（6.52士5.78）nmol/ml、（20.35士14.56）ng/ml,两组比较差异均有有统计学意义（P0.05）。SOD活性煤工尘肺组(86.54士19.85) U/ml，明显低于健康人群（104.87士18.08）U/ml,两组比较差异有统计学意义（P0.05）结论该研究发现IL-12B+1188A/C位点C等位基因可能是宁夏汉族煤工尘肺的易感基因之一。IL-6（-634C/G)位点多态性与宁夏地区煤工尘肺病的发生可能有关。IL-1β基因511位点多态性与宁夏地区煤工尘肺病的发生可能无关。宁夏煤工尘肺患者的MDA含量增高，SOD活力降低，细胞因子TGF-β1的表达增高，可能与煤工尘肺的发生发展有关。
[Abstract]:Objective to study the relationship between interleukin-12B (1188A/C), interleukin-6 (634C/G), interleukin-1 尾 (511C/T) gene polymorphisms and (CWP) in coal workers' pneumoconiosis in Ningxia Han nationality. The serum levels of transforming growth factor- 尾 1 (TGF- 尾 1), malondialdehyde (MDA) (MDA) and superoxide dismutase (SOD) in patients with pneumoconiosis were determined to explore the role and significance of transforming growth factor 尾 1 (TGF- 尾 1) and superoxide dismutase (SOD) in the pathogenesis of coal worker pneumoconiosis. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to analyze the polymorphism of IL-12B gene 1188A/C locus, IL-6 634C/G locus and IL-1 尾 511C/T locus in 120 coal worker pneumoconiosis patients and 120 dust exposed controls. The activity of MDA and the content of TGF- 尾 1 were determined by thiobarbituric acid (TBA) colorimetry, xanthine oxidase assay and enzyme linked immunosorbent assay (EL ISA) in 30 coal worker pneumoconiosis patients and 40 normal controls. Results the genotypes of IL-12B,IL-6,IL-1 尾 polymorphic loci in the two groups were in accordance with the Hardy-weinberg equilibrium and the length of service. There was no significant difference in job type distribution and smoke age between the coal worker pneumoconiosis group and the dust exposed control group (P > 0. 05). Three genotypes of AA,AC,CC were detected in the coal worker pneumoconiosis group and the dust exposure control group at locus 1188 of IL-12B gene. There was no significant difference in the distribution of these three genotypes between the coal worker pneumoconiosis group and the dust-exposed control group (P > 0.05), and the proportion of allele C in the coal worker pneumoconiosis group and the dust exposure control group was 45.8% and 36.7%, respectively (P < 0.05). There was no significant difference in the distribution of IL-1 尾 (C-511T) genotypes between the coal worker pneumoconiosis group and the dust exposed control group (P > 0. 05), but there was no significant difference between the coal worker pneumoconiosis group and the dust exposure control group (P > 0. 05). The distribution frequency of CC and CG GG at 634 locus of IL-6 gene in coal worker pneumoconiosis group (66.7%) was significantly higher than that in dust-exposed control group (52.5% 47.5%). There was no significant difference in the distribution of alleles between the two groups (P > 0.05). The content of MDA,TGF- 尾 1 in serum of coal worker pneumoconiosis patients was (41.61 卤33.35) nmol/ml, (27.76 卤13.86) ng/ml, significantly higher than that of healthy people (6.52 卤5.78) nmol/ml, (20.35 卤14.56) ng/ml,. There were significant differences between the two groups (P0.05). Sod activity in coal worker pneumoconiosis group (86.54 卤19.85) was significantly lower than that in healthy group (104.87 卤19.85). 18.08) U / ml, there was significant difference between the two groups (P0.05) conclusion this study found that the C allele of IL-12B 1188A/C locus may be one of the susceptible genes of coal worker pneumoconiosis in Ningxia Han nationality. IL-6 (-634C / G) polymorphism and the incidence of coal worker pneumoconiosis in Ningxia area. The 511 polymorphism of the. IL-1 尾 gene may not be related to the occurrence of coal worker pneumoconiosis in Ningxia. The increase of MDA content and the expression of cytokine TGF- 尾 1 in coal worker pneumoconiosis patients in Ningxia may be related to the occurrence and development of coal worker pneumoconiosis.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R135.2

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