3种方法治疗肠道菌群失调合并过敏性哮喘大鼠对肠道菌群的影响

发布时间：2018-10-20 11:19

【摘要】：目的根据"肺与大肠相表里"的理论,为肺肠合治法治疗肠道菌群失调并过敏性哮喘提供理论依据。方法实验共分了空白组、模型组、阳性组和3种中药复方的给药组;采用头孢哌酮加白色念珠菌灌胃SD大鼠的方式复制肠道菌群失调,并在此基础上运用卵清清蛋白注射致敏与雾化诱发哮喘,雾化同时分别给予加味升降散、桂枝加厚朴杏子汤和增液承气汤药液从肺肠合治、治肺和治肠进行治疗;采用Buxco全身体积描记系统分析呼吸功能,通过16SrDNA分析肠道菌群的变化。结果根据OTU值分析各组肠道菌群物种组成发现,各组对乳酸杆菌的恢复接近空白组且优于阳性组,3个治疗组均可促进变形菌门和拟杆菌门菌群的恢复,但肺肠合治效果最佳,通过Alpha多样性分析发现肠道微生物菌群多样性从高到低分别是治肠组、治肺组、肺肠合治组、模型组;呼吸频率和气道高反应性模型组为260.56(1.28),治疗后肺肠合治组、治肺组和治肠组分别为141.79(0.93)、137.25(1.30)和203.16(0.84),各治疗组大鼠呼吸频率均低于模型组,而气道高反应性只有肺肠合治组和治肠组低于模型组且差异有统计学意义(P0.05)。结论肺肠合治法治疗肠道菌群失调合并过敏性哮喘大鼠在肺功能和肠道菌群恢复上优于单纯治肠或治肺法。
[Abstract]:Objective to provide a theoretical basis for the treatment of intestinal dysbacteriosis and allergic asthma according to the theory of "lung and large intestine". Methods the rats were divided into blank group, model group, positive group and three kinds of traditional Chinese medicine compound administration group, the intestinal flora was duplicated by cefoperazone plus Candida albicans in SD rats. On this basis, asthma was induced by injection of ovalbumin to sensitize and atomize the asthma. Meanwhile, it was treated by adding modified Shengsheng San, Guizhi and Houpu Xingzi decoction and Zengye Chengqi decoction from lung to intestine, and treated with lung and intestine respectively. Respiratory function was analyzed by Buxco system and intestinal flora was analyzed by 16SrDNA. Results according to the analysis of the species composition of intestinal flora in each group, the recovery of Lactobacillus in each group was close to blank group and better than that in positive group. All the three treatment groups could promote the recovery of Proteus and Bacteroides, but the effect of lung intestinal colocalization was the best. Alpha diversity analysis showed that the diversity of intestinal microflora from high to low were treated group, lung combined group, model group, respiratory frequency and airway hyperresponsiveness group were 260.56 (1.28). 141.79 (0.93), 137.25 (1.30) and 203.16 (0.84), respectively. The respiratory frequency of each treatment group was lower than that of the model group, while the airway hyperresponsiveness was only lower in the lung combined group and the treated group than in the model group (P0.05). Conclusion the treatment of intestinal dysbacteriosis combined with allergic asthma is superior to that of simple intestinal therapy or lung therapy in the recovery of pulmonary function and intestinal flora in rats with allergic asthma.
【作者单位】：成都中医药大学;
【基金】：国家自然科学基金资助项目(81303085) 成都中医药大学科技发展基金资助项目(ZRYY1620)
【分类号】：R562.25

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