骨髓间充质干细胞移植联合吡菲尼酮治疗肺间质纤维化的研究

发布时间：2018-10-24 14:04

【摘要】：目的探讨吡菲尼酮(PFD)联合骨髓间充质干细胞(MSCs)移植入博来霉素(BLM)诱导肺纤维化小鼠的疗效。方法 36只健康清洁级体重200 g雄性SD大鼠,随机分为6组,A组为阴性对照组、B组阳性对照组、C组单纯激素治疗组、D组单纯MSCs移植组、E组单纯PFD治疗组、F组PFD联合MSCs移植组,各6只。SD大鼠体外培养MSCs,注入BLM制备肺纤维化模型,观察肺组织形态学变化,测定转化生长因子(TGF-β1)的蛋白表达。结果造模7d后,C、D、E、F组大鼠出现炎症细胞浸润比B组减少,肺纤维化程度明显减轻,F组较C、D、E组的病理改变更轻,肺纤维化不明显。肺系数中B组下降明显,与A组比较差异有统计学意义(P0.05)。B、C、D、E、F组肺纤维化评分高于A组,差异有统计学意义(P0.05)。肺组织中转化生长因子胶原表达的阳性细胞数明显减少,F组较B、C、D、E组数量减少和强度减弱更加明显,A组与B、C、D、E、F组比较,差异有统计学意义(P0.05),C、D、E组间差异无统计学意义(P0.05)。结论骨髓间充质干细胞移植联合毗菲尼酮对肺纤维化有一定的抑制作用,能够降低肺组织转化生长因子的表达,有修复和再生作用,治疗肺纤维化效果明显。
[Abstract]:Objective to investigate the effect of pifeinone (PFD) combined with bone marrow mesenchymal stem cell (MSCs) transplantation into bleomycin (BLM) induced pulmonary fibrosis in mice. Methods Thirty-six healthy, clean weight 200g male SD rats were randomly divided into 6 groups: group A was negative control group, group B was positive control group, group C was treated with hormone alone, group D was treated with MSCs, group E was treated with PFD alone, group F was treated with MSCs. Six SD rats were cultured with MSCs, in vitro to make pulmonary fibrosis model. The changes of lung histomorphology and the expression of TGF- 尾 1 protein were observed. Results after 7 days, the inflammatory cell infiltration was decreased and the degree of pulmonary fibrosis was alleviated. The pathological changes in group F were lighter than those in group C, and the pulmonary fibrosis was not obvious. The pulmonary coefficient of group B was significantly lower than that of group A (P0.05). The score of pulmonary fibrosis in group B was higher than that in group A (P0.05). The number of positive cells of transforming growth factor collagen expression in lung tissue was significantly decreased, and the number and intensity of TGF- collagen positive cells in group F was significantly lower than that in group B (P 0.05). The difference between group A and group F was statistically significant (P0.05), but there was no significant difference between group C and C (P0.05). Conclusion Bone marrow mesenchymal stem cell transplantation combined with pifinidone can inhibit pulmonary fibrosis, reduce the expression of transforming growth factor in lung tissue, repair and regenerate, and have obvious effect in the treatment of pulmonary fibrosis.
【作者单位】：赣南医学院第一附属医院;
【分类号】：R563

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