Anti-HBEC和ACA与慢性阻塞性肺疾病的相关性研究
发布时间：2018-10-25 17:45
【摘要】：第一章:慢性阻塞性肺疾病中Ig G、Ig A和Ig M型抗支气管上皮细胞抗体背景:近年来,越来越多的研究表明,自身免疫和支气管上皮细胞受损在慢性阻塞性肺疾病(COPD)的发病机制中起重要作用。循环的抗支气管上皮细胞抗体(anti-HBEC)结合到COPD患者的支气管上皮细胞上,可引起支气管上皮细胞的损伤。因此,本文旨在探讨COPD患者血清中Ig A、Ig G和Ig M型anti-HBEC与COPD临床特点的关系。方法:用间接免疫荧光的方法对170例COPD患者和150例健康对照组血清进行Ig A、Ig G和Ig M型anti-HBEC的检测,分析该抗体阳性与COPD患者的肺功能、BMI、性别和年龄等的关系。结果:COPD中Ig G型anti-HBEC阳性率明显高于健康对照组(20.00%vs7.33%,1:100稀释)(P0.05);Ig A型anti-HBEC阳性率也显著高于健康对照组(29.41%vs 8.67%,1:40稀释)(P0.001);Ig M型anti-HBEC阳性率高于对照组,但是无显著性差异(11.18%vs 6.67%,1:40稀释)(P0.05)。Ig G和Ig A型anti-HBEC阳性的COPD患者主要集中在GOLD III期和GOLD IV期,并且Ig A型anti-HBEC阳性的COPD患者BMI偏低(P0.05)。结论:Ig G和Ig A型anti-HBEC与COPD的GOLD分期有关联。因此本研究表明,Ig G和Ig A型anti-HBEC参与晚期部分COPD的发病,可能会作为评估COPD严重性的新型标志物。第二章:慢性阻塞性肺疾病抗心磷脂抗体水平分析目的:探讨抗心磷脂抗体(ACA)与慢性阻塞性肺疾病(COPD)的关系。方法:采用酶联免疫吸附试验(ELISA)的方法检测了103例COPD患者与72例健康对照者血清中的ACA Ig G/A/M,将COPD组103例分成ACA阳性和阴性两组,比较ACA阳性和阴性与患者的性别、年龄、体重指数(BMI)和肺功能指标之间的关系。结果:从定性角度,在COPD组和健康对照组中,ACA的阳性率分别为16.5%(17/103)和4.2%(3/72),两者之间差异有显著性的统计学意义(P0.05);在COPD的慢性阻塞性肺疾病全球倡议(GOLD)分期中,从GOLD I到GOLDⅣ期,ACA的阳性率依次为66.7%,18.5%,13.9%,3.2%,逐渐降低。从定量角度,COPD组ACA浓度为4.4±0.7 RU/ml,远高于健康对照组的2.6±0.4 RU/ml(P0.05);其中,从GOLD I到GOLDⅣ期,ACA浓度依次为10.9±2.6 RU/ml、3.9±1.0 RU/ml、4.8±1.4RU/ml、2.5±0.6 RU/ml。ACA阳性的COPD患者主要集中在GOLD I期,GOLD I期的ACA阳性率和浓度远高于其它各期。其中,ACA阳性与COPD患者的相关临床指标没有明显的关联(P0.05)。结论:ACA与COPD的GOLD分期有关联,主要出现在疾病早期。因此本研究表明,ACA可能会作为诊断早期COPD的一个新型标志物,并在COPD的发病机制中起到一定作用。
[Abstract]:Chapter 1: background of Ig G A and Ig M anti bronchial epithelial cell antibodies in chronic obstructive pulmonary disease: in recent years, more and more studies have shown that, Autoimmune and bronchial epithelial cell damage play an important role in the pathogenesis of chronic obstructive pulmonary disease (COPD). Circulating anti-bronchial epithelial cell antibodies (anti-HBEC) bind to the bronchial epithelial cells of COPD patients and can cause the injury of bronchial epithelial cells. Therefore, the purpose of this study was to investigate the relationship between serum Ig Ag G and Ig M anti-HBEC and the clinical characteristics of COPD in patients with COPD. Methods: indirect immunofluorescence was used to detect Ig Ag G and Ig M anti-HBEC in 170 patients with COPD and 150 healthy controls. The relationship between the positive antibody and pulmonary function, BMI, sex and age of COPD patients was analyzed. Results: the positive rate of Ig G type anti-HBEC in COPD was significantly higher than that in the healthy control group (20.00 vs 7.33 vs 1: 100 dilution) (P0.05); Ig A anti-HBEC positive rate was also significantly higher than that in the healthy control group (29.41%vs 8.67 + 1: 40 dilution) (P0.001); Ig M anti-HBEC positive rate was higher than that in the control group). But there was no significant difference (11.18%vs 6.67% dilution) (P0.05). Ig G and Ig A anti-HBEC positive COPD patients were mainly in GOLD III and GOLD IV phase, and Ig A anti-HBEC positive COPD patients BMI was lower (P0.05). Conclusion: Ig G and Ig A anti-HBEC are associated with the GOLD staging of COPD. Therefore, this study suggests that, Ig G and Ig A type anti-HBEC may be a new marker for evaluating the severity of COPD. Chapter 2: analysis of anticardiolipin antibody level in chronic obstructive pulmonary disease objective: to investigate the relationship between anticardiolipin antibody (ACA) and chronic obstructive pulmonary disease (COPD). Methods: the serum ACA Ig G / A / M of 103 patients with COPD and 72 healthy controls were detected by enzyme linked immunosorbent assay (ELISA). The patients in COPD group were divided into two groups: ACA positive group and ACA negative group. The sex and age of ACA positive and negative patients were compared. Relationship between body mass index (BMI) and pulmonary function. Results: qualitatively, the positive rates of ACA in COPD group and healthy control group were 16.5% (17 / 103) and 4.2% (3 / 72), respectively. The difference was statistically significant (P0.05). From GOLD I to GOLD 鈪，
本文编号：2294375
[Abstract]:Chapter 1: background of Ig G A and Ig M anti bronchial epithelial cell antibodies in chronic obstructive pulmonary disease: in recent years, more and more studies have shown that, Autoimmune and bronchial epithelial cell damage play an important role in the pathogenesis of chronic obstructive pulmonary disease (COPD). Circulating anti-bronchial epithelial cell antibodies (anti-HBEC) bind to the bronchial epithelial cells of COPD patients and can cause the injury of bronchial epithelial cells. Therefore, the purpose of this study was to investigate the relationship between serum Ig Ag G and Ig M anti-HBEC and the clinical characteristics of COPD in patients with COPD. Methods: indirect immunofluorescence was used to detect Ig Ag G and Ig M anti-HBEC in 170 patients with COPD and 150 healthy controls. The relationship between the positive antibody and pulmonary function, BMI, sex and age of COPD patients was analyzed. Results: the positive rate of Ig G type anti-HBEC in COPD was significantly higher than that in the healthy control group (20.00 vs 7.33 vs 1: 100 dilution) (P0.05); Ig A anti-HBEC positive rate was also significantly higher than that in the healthy control group (29.41%vs 8.67 + 1: 40 dilution) (P0.001); Ig M anti-HBEC positive rate was higher than that in the control group). But there was no significant difference (11.18%vs 6.67% dilution) (P0.05). Ig G and Ig A anti-HBEC positive COPD patients were mainly in GOLD III and GOLD IV phase, and Ig A anti-HBEC positive COPD patients BMI was lower (P0.05). Conclusion: Ig G and Ig A anti-HBEC are associated with the GOLD staging of COPD. Therefore, this study suggests that, Ig G and Ig A type anti-HBEC may be a new marker for evaluating the severity of COPD. Chapter 2: analysis of anticardiolipin antibody level in chronic obstructive pulmonary disease objective: to investigate the relationship between anticardiolipin antibody (ACA) and chronic obstructive pulmonary disease (COPD). Methods: the serum ACA Ig G / A / M of 103 patients with COPD and 72 healthy controls were detected by enzyme linked immunosorbent assay (ELISA). The patients in COPD group were divided into two groups: ACA positive group and ACA negative group. The sex and age of ACA positive and negative patients were compared. Relationship between body mass index (BMI) and pulmonary function. Results: qualitatively, the positive rates of ACA in COPD group and healthy control group were 16.5% (17 / 103) and 4.2% (3 / 72), respectively. The difference was statistically significant (P0.05). From GOLD I to GOLD 鈪，
本文编号：2294375
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