煤矿工人血清趋化因子MIP-1α、MCP-1和IL-8表达及意义

发布时间：2018-11-01 13:00

【摘要】：目的检测煤矿工人血清趋化因子水平,以探索敏感、特异的煤工尘肺(CWP)早期识别和诊断指标。方法采用随机抽样方法,以某煤矿180名诊断为粉尘作业观察对象(肺部影像学改变未达到CWP壹期程度)的工人为观察组,并分别以该煤矿胸部X射线检查结果正常的180名接尘工人和161名非接尘工人为接尘组和对照组。3组人群均根据吸烟史分别划分为吸烟亚组和非吸烟亚组。采集3组人员空腹静脉血,酶联免疫吸附法测定血清趋化因子巨噬细胞炎症蛋白-1α(MIP-1α)、单核细胞趋化蛋白-1(MCP-1)和白细胞介素-8(IL-8)的水平。结果观察组MIP-1α、MCP-1和IL-8表达水平分别高于接尘组和对照组(P0.05),接尘组上述3个指标表达水平均高于对照组(P0.05)。观察组吸烟亚组3个指标表达水平分别高于对照组吸烟亚组和接尘组吸烟亚组,接尘组吸烟亚组3个指标表达水平高于对照组吸烟亚组(P0.05)。观察组非吸烟亚组3个指标表达水平均高于对照组非吸烟亚组,观察组非吸烟亚组MIP-1α和IL-8表达水平均高于接尘组非吸烟亚组(P0.05)。在排除年龄、吸烟和组别3个混杂因素后,血清IL-8表达水平与接尘工龄呈正相关(P0.05);但血清MIP-1α和MCP-1表达水平均与接尘工龄不相关(P0.05)。结论 CWP早期血清中MIP-1α、MCP-1和IL-8已有差异性表达,这可能促进CWP的发生与发展。建议将上述3个指标作为CWP的早期识别和早期诊断的生物标志物。
[Abstract]:Objective to detect the level of serum chemokines in coal miners in order to explore sensitive and specific early identification and diagnosis indexes of coal workers' pneumoconiosis by (CWP). Methods A random sampling method was used to observe 180 workers who were diagnosed as dust workers in a coal mine (lung imaging changes were not up to stage I of CWP). 180 workers exposed to dust and 161 workers who were not exposed to dust were divided into smoking subgroup and non-smoking subgroup according to smoking history. Fasting venous blood was collected and the levels of chemokine macrophage inflammatory protein-1 伪 (MIP-1 伪), monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) were measured by enzyme-linked immunosorbent assay (Elisa). Results the expression levels of MIP-1 伪, MCP-1 and IL-8 in the observation group were higher than those in the dust-exposed group and the control group (P0.05), and the expression levels of the above three indexes in the dust-exposed group were higher than those in the control group (P0.05). The expression level of three indexes in smoking subgroup of observation group was higher than that of smoking subgroup of control group and dust-exposed group, and the expression level of three indexes in smoking subgroup of dust-exposed group was higher than that of smoking subgroup of control group (P0.05). The expression levels of MIP-1 伪 and IL-8 in the observation group were higher than those in the control group (P0.05), and the expression levels of MIP-1 伪 and IL-8 in the observation group were higher than those in the non-smoking subgroup (P0.05). Serum IL-8 expression was positively correlated with exposure to dust (P0.05), but serum MIP-1 伪 and MCP-1 levels were not correlated with length of exposure to dust (P0.05). Conclusion the differential expression of MIP-1 伪, MCP-1 and IL-8 in the early stage of CWP may promote the occurrence and development of CWP. It is suggested that the above three indexes be used as biomarkers for early identification and early diagnosis of CWP.
【作者单位】：河北联合大学公共卫生学院;开滦职业病防治院;新乡医学院公共卫生学院;
【基金】：国家自然科学基金(81273017) 河北省重点职业病防治技术研究(13277709D)
【分类号】：R135.2

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