黄芩黄酮与维生素E对染尘小鼠肺损伤的保护作用

发布时间：2018-11-21 07:39

【摘要】：目的探讨黄芩黄酮与维生素E单独与联合应用对染尘小鼠肺损伤过程中SOD活性、MDA、LDH以及Hyp含量的影响。方法采用气管注入法给予二氧化硅混悬液(SiO2)建立染尘鼠模型。建模1周后按照2×2析因设计将实验动物分为模型对照组A,黄芩黄酮干预组B,维生素E干预组C,黄芩黄酮与维生素E联合干预组D,并设正常对照组N。给药2周后分别测定各组的SOD活性、MDA、LDH以及Hyp的含量。结果与模型对照组比较,黄芩黄酮组、维生素E组、黄芩黄酮与维生素E联合组均能提高SOD活性,可有效减少MDA的产生(P0.05),黄芩黄酮与维生素E联合组的效果较单独应用效果明显;黄酮黄芩与维生素E联合能明显减少Hyp、LDH的含量及肺体重指数(P0.05)。结论黄芩黄酮与维生素E有交互作用,能提高小鼠SOD的活性,减少LDH、脂质过氧化反应物以及Hyp的生成,对肺损伤有更好的保护作用。
[Abstract]:Objective to investigate the effects of flavonoids of Scutellaria baicalensis and vitamin E on SOD activity, MDA,LDH and Hyp contents during lung injury in dust-exposed mice. Methods silica suspension (SiO2) was injected into trachea to establish the model of dust-infected mice. After 1 week of modeling, the experimental animals were divided into three groups according to 2 脳 2 factorial design: model control group (A), baicalein intervention group (B), vitamin E intervention group (C), baicalein and vitamin E combined intervention group (D), and normal control group (N). The activity of SOD, the content of MDA,LDH and Hyp were measured 2 weeks after administration. Results compared with the model control group, baicalein group, vitamin E group, baicalein group and vitamin E group all increased the activity of SOD and effectively reduced the production of MDA (P0.05). The effect of Scutellaria flavone combined with vitamin E was better than that of single application. The combination of flavonoid baicalin and vitamin E could significantly reduce the content of Hyp,LDH and lung body mass index (P0.05). Conclusion flavonoids of Scutellaria baicalensis have interaction with vitamin E, which can increase the activity of SOD, reduce the production of LDH, lipid peroxidation and Hyp, and have better protective effect on lung injury.
【作者单位】：承德医学院预防医学教研室;
【基金】：河北省卫生厅科研基金项目(20130391)
【分类号】：R135.2

【参考文献】