尘肺患者血清miR-21的表达及其与临床分期的关系

发布时间：2018-11-28 12:15

【摘要】：目的检测不同临床分期尘肺患者血清中miR-21的表达水平,探讨其作为尘肺分子标志物的可能性。方法采集118例男性尘肺患者(Ⅰ期57例、Ⅱ期48例和Ⅲ期13例)静脉血标本,收集血清,用实时荧光定量RT-PCR法检测其miR-21的表达水平,并以115例年龄、性别匹配的健康人作为对照。结果健康对照者血清miR-21的相对表达量为(0.004±0.003),Ⅰ期和Ⅱ~Ⅲ期尘肺患者分别为(0.011±0.008)和(0.022±0.018)。Ⅰ期和Ⅱ~Ⅲ期尘肺患者血清miR-21水平显著高于健康对照组(t分别为-6.39,-7.81,P均0.01)。且Ⅱ~Ⅲ期患者高于Ⅰ期(t=-4.28,P0.01)。用于尘肺诊断时miR-21的ROC曲线下面积(AUCROC)为0.85(95%CI:0.78~0.93),以相对表达量0.007为临界值,诊断尘肺的敏感性和特异性分别为83.9%和90.4%。结论 miR-21在尘肺患者血清中高表达,且与临床分期有关,有望成为用于尘肺诊断的分子标志物。
[Abstract]:Objective to detect the expression of miR-21 in serum of patients with pneumoconiosis at different clinical stages and to explore the possibility of using it as a molecular marker of pneumoconiosis. Methods the venous blood samples were collected from 118 male patients with pneumoconiosis (57 cases in stage 鈪，

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