姜黄素对肺血栓栓塞症大鼠肺组织不规则趋化因子的影响

发布时间：2018-12-05 21:07

【摘要】：目的:探索肺血栓栓塞症(pulmonary thromboembolism,PTE)大鼠肺组织不规则趋化因子(Fractalkine,FKN)的变化及姜黄素(curcumin,CUR)的干预作用。方法:采用自体血栓法复制PTE动物模型,96只SD大鼠随机分6组:空白组组、假手术组、模型组、CUR低剂量组、CUR中剂量组、CUR高剂量组,栓塞后4h、72h,分别检测血清TNF-α、IL-1β、IL-8、FKN,荧光定量PCR检测肺组织TNF-α、IL-8、FKN的mRNA水平,肺病理HE染色,免疫组化染色检测CX3CL1及CX3CR1阳性细胞数。结果:栓塞后4h血清ELISA的FKN比较:空白组显著小于模型组(P0.01),72h后空白组、假手术组、CUR高剂量组均显著小于模型组(P0.01);栓塞后4h、72h IL-8比较:空白组、假手术组、CUR低剂量组、CUR中剂量组显著小于模型组(P0.05);栓塞后72h血清ELISA的TNF-α比较:空白组、假手术组、CUR高剂量组均显著小于模型组(P0.05);栓塞后72h各组血清ELISA的IL-1β均显著小于模型组(P0.05)。各组栓塞后4h和72h肺组织FKN、TNF-α、IL-1β的mRNA均显著小于模型组(P0.01)。栓塞后72h大鼠肺HE染色呈肺泡壁血管高度扩张、充血,个别大鼠光镜下查见出血性梗死灶,应用CUR后肺组织充血程度明显减轻,出现梗死的大鼠数量也有降低的趋势。免疫组化发现栓塞后4h,空白组、假手术组、CUR高剂量组的CX3CL1阳性细胞计数均显著小于模型组(P0.01),空白组、假手术组、CUR中剂量组、CUR高剂量组的CX3CR1阳性细胞计数显著小于模型组(P0.01)。栓塞后72h,空白组、假手术组、CUR低剂量组、CUR中剂量组、CUR高剂量组的CX3CL1阳性细胞计数均显著小于模型组(P0.05),空白组、假手术组的CX3CR1阳性细胞计数显著小于模型组(P0.01)。结论:PTE大鼠存在炎性因子,CUR能抑制PTE大鼠肺TNF-α、IL-1β、IL-8、CX3CL1及CX3CR1的表达,可能是CUR减轻PTE大鼠肺损伤的机制之一。
[Abstract]:Aim: to investigate the changes of irregular chemokine (Fractalkine,FKN) in pulmonary tissue and the intervention of curcumin (curcumin,CUR) in pulmonary thromboembolism (pulmonary thromboembolism,PTE) rats. Methods: PTE animal model was established by autologous thrombosis. 96 SD rats were randomly divided into 6 groups: blank group, sham operation group, model group, CUR low dose group, CUR medium dose group, CUR high dose group, 4 h after embolization and 72 h after embolization. Serum TNF- 伪, IL-1 尾 and IL-8,FKN, fluorescence quantitative PCR were used to detect the mRNA levels of TNF- 伪 and IL-8,FKN in lung tissue, HE staining in lung pathology, and CX3CL1 and CX3CR1 positive cells in lung tissue by immunohistochemical staining. Results: the comparison of FKN of serum ELISA 4 hours after embolization: the blank group was significantly smaller than the model group (P0.01), the blank group after 72 hours, the sham operation group, the high dose CUR group were significantly smaller than the model group (P0.01). Comparison of IL-8 at 72h after embolization: blank group, sham operation group, CUR low dose group, CUR medium dose group were significantly lower than model group (P0.05); Comparison of TNF- 伪 of serum ELISA at 72 hours after embolization: the IL-1 尾 of serum ELISA in blank group, sham operation group and high dose group of CUR were significantly lower than that in model group (P0.05), and IL-1 尾 in serum of 72 h after embolization was significantly lower than that in model group (P0.05). The mRNA of FKN,TNF- 伪 and IL-1 尾 in lung tissue of each group was significantly lower than that of model group at 4h and 72h after embolization (P0.01). At 72 hours after embolization, the pulmonary HE staining showed high dilation of alveolar wall vessels and congestion. The hemorrhagic infarct was found in some rats under light microscope. The degree of congestion in lung tissue was obviously reduced after the application of CUR, and the number of rats with infarction also decreased. Immunohistochemistry showed that the number of CX3CL1 positive cells in the blank group, sham operation group, high dose CUR group was significantly lower than that in the model group (P0.01), blank group, sham-operation group, CUR medium dose group, the number of CX3CL1 positive cells was significantly lower than that in the model group (P0.01). The number of CX3CR1 positive cells in CUR group was significantly lower than that in model group (P0.01). At 72 hours after embolization, the counts of CX3CL1 positive cells in blank group, sham operation group, CUR low dose group, CUR medium dose group and CUR high dose group were significantly lower than those in model group (P0.05). The number of CX3CR1 positive cells in sham-operated group was significantly lower than that in model group (P0.01). Conclusion: there are inflammatory factors in PTE rats, and CUR can inhibit the expression of TNF- 伪, IL-1 尾, IL-8,CX3CL1 and CX3CR1 in the lung of PTE rats, which may be one of the mechanisms that CUR attenuates the lung injury of PTE rats.
【学位授予单位】：浙江中医药大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R563.5

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