免疫抑制并铜绿假单胞菌肺炎小鼠Th17细胞功能的变化及人免疫球蛋白治疗对其的影响

发布时间：2019-01-05 15:09

【摘要】：目的探讨辅助性T细胞17(Th17)的转录调节因子——核孤儿受体γt(RORγt)及主要效应分子白细胞介素-17(IL-17)在免疫抑制并铜绿假单胞菌(PA)肺炎中的变化,以此反映Th17细胞功能变化,以及人免疫球蛋白治疗对其的影响。方法 90只BALB/c小鼠分为空白组、PA组、免疫抑制组、免疫抑制并PA组及人免疫球蛋白治疗组(分别记为A、B、C、D、E组),每组18只。各组小鼠于造模成功后的4、8及24h处死,观察小鼠大体病理改变,进行肺组织病理学评分,反转录-聚合酶链式反应检测肺组织RORγt mRNA表达,酶联免疫法检测外周血中IL-17表达水平。结果光镜下可见,A、C组小鼠肺组织无明显炎性表现,B、D、E组小鼠肺组织均可见不同程度的组织充血、水肿、炎性细胞浸润、肺泡间隔增宽等炎性改变,均以8h最重,其中D组小鼠肺组织病理评分明显高于其它各组(均P0.01);B、D、E组小鼠肺组织中RORγt mRNA表达水平较A、C明显上调(均P0.01),并均以4h达峰值;B、D、E组小鼠外周血中IL-17表达水平较A、C组明显升高(均P0.01),均于8h达峰值。D组相比于B组,病理评分、RORγt mRNA及IL-17表达水平均增高(均P0.01),而E组则较B组均降低(均P0.01)。结论免疫抑制并铜绿假单胞菌肺炎中Th17细胞过度激活,引起过度炎症反应导致组织器官损伤,而人免疫球蛋白可在一定程度上抑制Th17细胞为主的炎症反应,从而减缓此类疾病病情的发展。
[Abstract]:Objective to investigate the changes of nuclear orphan receptor 纬 t (ROR 纬 t, a transcription regulator of helper T cell 17 (Th17), and the main effector molecule interleukin-17 (IL-17) in immunosuppressive and Pseudomonas aeruginosa (PA) pneumonia. This reflects the changes of Th17 cell function and the effect of human immunoglobulin therapy on it. Methods 90 BALB/c mice were divided into blank group, PA group, immunosuppressive PA group and human immunoglobulin group (18 rats in each group). The mice in each group were killed at 4 h and 24 h after the successful establishment of the model. The gross pathological changes of the mice were observed, the lung histopathology score and the expression of ROR 纬 t mRNA in the lung tissue were detected by reverse transcription-polymerase chain reaction (RT-PCR). The expression of IL-17 in peripheral blood was detected by enzyme-linked immunosorbent assay (Elisa). Results under the light microscope, there was no obvious inflammatory manifestation in lung tissue of ANAC group. In group B, there were different degree of congestion, edema, infiltration of inflammatory cells, widening of alveolar septum and other inflammatory changes, all of which were most serious at 8 h. The pathological score of lung tissue in group D was significantly higher than that in other groups (P0.01). The expression level of ROR 纬 t mRNA in the lung tissue of group B was significantly higher than that in group A (P0.01), and reached its peak at 4 h. The expression of IL-17 in peripheral blood of group B was significantly higher than that of group A (P0.01), and reached its peak at 8 h. Compared with group B, the expression of ROR 纬 t mRNA and IL-17 in group D was significantly higher than that in group B (P0.01). Group E was lower than group B (P 0.01). Conclusion the excessive activation of Th17 cells in immunosuppressive and Pseudomonas aeruginosa pneumonia may lead to excessive inflammation and injury of tissues and organs, while human immunoglobulin can inhibit the inflammatory response of Th17 cells to some extent. Thus slowing down the development of such diseases.
【作者单位】：郑州大学第一附属医院呼吸ICU(RICU);郑州大学基础医学院微生物与免疫学教研室;郑州大学第一附属医院检验科;
【基金】：河南省科技厅基础与前沿技术研究资助项目(No.142300410327) 河南省医学科技攻关计划资助项目(No.201403060)
【分类号】：R563.1

【相似文献】