白介素18和Treg细胞与慢性阻塞性肺疾病的相关性研究

发布时间：2019-01-06 16:21

【摘要】：目的探讨吸烟慢性阻塞性肺疾病(COPD)稳定期患者、健康吸烟者及健康非吸烟者外周血中白介素(IL)-18及Treg细胞变化及其临床意义。方法选择2012年11月—2013年5月于河北省人民医院呼吸内科门诊就诊的吸烟COPD患者33例为吸烟COPD组,体检中心体检健康者分为健康吸烟组19例和健康非吸烟组16例。采用酶联免疫吸附测定(ELISA)法检测受试者外周血中IL-18、IL-17水平,用流式细胞术检测CD8+及Treg细胞比例。结果吸烟COPD组IL-18、IL-17、CD8+较健康吸烟组、健康非吸烟组均升高,Treg细胞较健康吸烟组下降、较健康非吸烟组升高(P0.05);健康吸烟组IL-18、IL-17、CD8+及Treg细胞较健康非吸烟组均升高(P0.05)。结论IL-18分泌增多,诱导IL-17、CD8+细胞上升,超出Treg细胞的抑制能力以及Treg细胞的反应钝化可能导致吸烟COPD患者气道的慢性炎症。
[Abstract]:Objective to investigate the changes and clinical significance of interleukin (IL) 18 and Treg cells in peripheral blood of smoking patients with chronic obstructive pulmonary disease (COPD), healthy smokers and non smokers. Methods from November 2012 to May 2013, 33 patients with smoking COPD in Department of Respiratory Medicine, Hebei Provincial people's Hospital were selected as COPD smoking group. The healthy subjects were divided into healthy smoking group (n = 19) and healthy non-smoking group (n = 16). The levels of IL-18,IL-17 in peripheral blood and the proportion of CD8 and Treg cells in peripheral blood were detected by Elisa and flow cytometry respectively. Results the IL-18,IL-17,CD8 of smoking COPD group was higher than that of healthy smoking group, the Treg cell of healthy non-smoking group was higher than that of healthy smoking group, and the Treg cell level was higher than that of healthy non-smoking group (P0.05). IL-18,IL-17,CD8 and Treg cells in healthy smoking group were higher than those in healthy non-smoking group (P 0.05). Conclusion the increase of IL-18 secretion and the increase of IL-17,CD8 cells may lead to chronic airway inflammation in patients with COPD smoking, which is more than the inhibition of Treg cells and the passivation of Treg cells.
【作者单位】：河北省人民医院呼吸内科;武安市人民医院;河北医科大学呼吸病系;
【基金】：2011年河北省医学科学研究重点课题项目(20110013)
【分类号】：R563.9

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