不同容量机械通气对盐酸诱导急性呼吸窘迫综合征大鼠肺损伤的作用机制

发布时间：2019-01-07 21:59

【摘要】：目的:研究不同容量机械通气对盐酸诱导急性呼吸窘迫综合征(ARDS)大鼠肺损伤的作用机制。方法:30只SD大鼠随机分为对照组、盐酸+高潮气量(HCL+HV)组及盐酸+低潮气量(HCL+LV)组,每组10只。HCL+LV组通气容量为6 mL/kg体重,通气末正压为3 cmH2O;HCL+HV组通气容量为15 mL/kg体重,通气末正压为0 cmH2O。两组大鼠连续通气6 h后与对照组一起处死,行肺泡灌洗并取肺组织进行病理观察;并用ELISA法检测3组肺组织中的相关指标。结果:模型组大鼠可见肺组织中ARDS病理改变;与对照组相比,两个模型组肺损伤积分、肺灌洗液中性细胞比例(PMN)、MIP-2、TNF-α均有统计学意义,其中HCL+HV组各指标明显高于HCL+LV组(均P0.01)。结论:HCL诱导的ARDS大鼠在机械通气情况下有典型的肺损伤的表现;低容量机械通气对ARDS大鼠的肺损伤有保护作用。
[Abstract]:Aim: to study the mechanism of different volume mechanical ventilation on lung injury induced by hydrochloric acid in acute respiratory distress syndrome (ARDS) rats. Methods: thirty SD rats were randomly divided into control group, (HCL HV) group with high volume of hydrochloric acid and (HCL LV) group with low tidal volume of hydrochloric acid. The ventilation volume of 10. HCL LV rats in each group was 6 mL/kg body weight and the positive end pressure of ventilation was 3 cmH2O;. The ventilation volume of HCL HV group was 15 mL/kg body weight and the positive end pressure of ventilation was 0 cmH2O.. The rats in the two groups were killed together with the control group after 6 hours of continuous ventilation. The alveolar lavage was performed and the lung tissues were taken for pathological observation. The relative indexes in the lung tissues of the three groups were detected by ELISA method. Results: the pathological changes of ARDS in lung tissue were observed in the model group. Compared with the control group, the score of lung injury and the ratio of neutrophils in lung lavage fluid (PMN), MIP-2,TNF- 伪) in the two model groups were significantly higher than those in the HCL LV group (P0.01), and each index in the HCL HV group was significantly higher than that in the HCL LV group (P0.01). Conclusion: ARDS rats induced by HCL have typical manifestations of lung injury under mechanical ventilation and low volume mechanical ventilation has protective effect on lung injury in ARDS rats.
【作者单位】：南方医科大学南方医院神经内科;
【分类号】：R563.8

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