高氧液对油酸性急性肺损伤家兔肺组织改变和肺表面活性物质的影响

发布时间：2019-01-11 14:15

【摘要】：目的观察高氧液对急性油酸性肺损伤家兔肺组织形态改变及肺表面活性物质的影响。方法健康家兔30只,雌雄不拘,体质量2.0~2.5kg,随机分为3组:空白对照组(n=10);高氧液治疗组(n=10);盐水对照组(n=10)。3组动物给予相应处理后分别在ALI模型制备前(基础值)、静脉输液前(损伤后)、输液后30min、1h、2h时观察动脉血气变化,2h后处死动物取肺组织做病理切片观察肺组织形态学改变和免疫组化检测肺表面活性物质的变化。结果高氧液治疗组在输注高氧液后2hPaO2明显高于盐水对照组(P0.001),而PaCO2则低于盐水对照组(P0.001)。光镜下高氧液治疗组家兔肺组织损伤程度较盐水对照组轻。免疫组化及肺泡灌洗液测定结果显示,高氧液治疗组肺表面活性物质A含量明显高于盐水对照组(P0.001)。结论静脉输注高氧液可减轻肺组织损伤程度,减少肺泡表面活性物质的破坏。
[Abstract]:Objective to observe the effects of hyperoxia on lung tissue morphology and pulmonary surfactant in rabbits with acute oily acid lung injury. Methods Thirty healthy rabbits, male and female, were randomly divided into three groups: blank control group (n = 10), hyperoxia solution group (n = 10) and hyperoxia solution group (n = 10). The changes of arterial blood gas were observed before ALI model preparation (basic value), before intravenous infusion (after injury) and 30 minutes after infusion for 1 hour and 2 hours after treatment. After 2 hours, the lung tissues were taken to observe the changes of lung morphology and the changes of pulmonary surfactant by immunohistochemistry. Results the 2hPaO2 of hyperoxia solution group was significantly higher than that of saline control group (P0.001), but PaCO2 was lower than that of saline control group (P0.001). The degree of lung injury in hyperoxia solution group was lighter than that in saline control group. The results of immunohistochemistry and alveolar lavage fluid test showed that the content of surfactant A in hyperoxia solution group was significantly higher than that in saline control group (P0.001). Conclusion intravenous infusion of hyperoxia solution can reduce the degree of lung tissue injury and reduce the destruction of alveolar surfactant.
【作者单位】：西安交通大学医学部第一附属医院麻醉科;西北妇女儿童医院麻醉科;
【基金】：陕西省科技攻关计划项目(No.2004K12-G11)~~
【分类号】：R563.8

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