Tfh细胞以及Bcl-6蛋白在哮喘小鼠中的表达以及氨茶碱的干预作用
发布时间：2019-01-18 17:11
【摘要】：目的研究哮喘小鼠体内Tfh细胞以及Bcl-6蛋白的变化,并初步探讨氨茶碱对哮喘的治疗作用。方法 4~6周龄BALB/c小鼠40只,随机分成4组,每组10只。分别为正常对照组、哮喘模型组、氨茶碱治疗组及地塞米松阳性对照组。后3组建立哮喘模型后对应给以基础处置。按两因素析因设计测取小鼠气道反应性资料,HE染色观察小鼠肺组织的病理形态,流式细胞术检测Tfh细胞的比例变化,Western-blotting检测Bcl-6的蛋白表达量,Elisa法测定肺泡灌洗液以及血清中IL-4、IL-10的水平。结果哮喘模型组小鼠的气道阻力较正常组小鼠显著升高(P0.05),HE染色切片中气道平滑肌增厚,且有大量的炎症细胞浸润,炎症评分较正常组明显升高(P0.05),小鼠脾脏中的Tfh细胞的比例较正常对照组小鼠显著升高,Bcl-6蛋白含量较正常组明显升高,血清以及肺泡灌洗液中IL-4含量较正常对照组显著升高,而IL-10的水平显著下降;而氨茶碱治疗组以及地塞米松阳性对照组的气道阻力较哮喘模型组小鼠显著降低(P0.05),小鼠HE染色切片发现其炎症细胞浸润程度较哮喘模型组显著降低,Tfh细胞的比例较哮喘模型组显著降低,肺组织中Bcl-6蛋白表达水平较哮喘模型组显著降低,血清以及肺泡灌洗液中IL-4含量较哮喘模型组明显下降,IL-10水平显著升高。结论哮喘小鼠Tfh细胞比例增加,Bcl-6蛋白水平升高,氨茶碱可以有效改善哮喘小鼠气道炎症和气道高反应性,可能与其影响Tfh细胞比例以及IL-4、IL-10的水平有关。
[Abstract]:Objective to study the changes of Tfh cells and Bcl-6 protein in asthmatic mice and to explore the therapeutic effect of aminophylline on asthma. Methods 40 BALB/c mice aged 4 weeks 6 weeks were randomly divided into 4 groups with 10 mice in each group. They were normal control group, asthma model group, aminophylline treatment group and dexamethasone positive control group. The asthmatic model was established in the latter three groups. The airway reactivity of mice was measured by two factor factorial design. HE staining was used to observe the pathological morphology of mouse lung tissue, flow cytometry was used to detect the proportion of Tfh cells, and Western-blotting was used to detect the protein expression of Bcl-6. The levels of IL-4,IL-10 in alveolar lavage fluid and serum were measured by Elisa method. Results the airway resistance in asthmatic model group was significantly higher than that in normal group (P0.05). The airway smooth muscle was thickened and a large number of inflammatory cells infiltrated in the), HE staining section, and the inflammatory score was significantly higher than that in the normal group (P0.05). The percentage of Tfh cells in the spleen of mice was significantly higher than that of the control group, the content of Bcl-6 protein was significantly higher than that of the normal group, and the content of IL-4 in serum and alveolar lavage fluid was significantly higher than that in the normal control group. However, the level of IL-10 decreased significantly. The airway resistance in the aminophylline treatment group and the dexamethasone positive control group was significantly lower than that in the asthma model group (P0.05). The degree of inflammatory cell infiltration in the HE staining section of the mice was significantly lower than that in the asthmatic model group. The proportion of Tfh cells was significantly lower than that of asthma model group, the expression of Bcl-6 protein in lung tissue was significantly lower than that in asthmatic model group, the content of IL-4 in serum and alveolar lavage fluid was significantly lower than that in asthma model group, and the level of IL-10 was significantly higher than that in asthmatic model group. Conclusion Aminophylline can effectively improve airway inflammation and airway hyperresponsiveness in asthmatic mice by increasing the proportion of Tfh cells and the level of Bcl-6 protein, which may be related to the effect of aminophylline on the proportion of Tfh cells and the level of IL-4,IL-10.
【作者单位】： 河北省唐山市工人医院呼吸科;河北联合大学附属医院呼吸内科;
【分类号】：R562.25
，
本文编号：2410929
[Abstract]:Objective to study the changes of Tfh cells and Bcl-6 protein in asthmatic mice and to explore the therapeutic effect of aminophylline on asthma. Methods 40 BALB/c mice aged 4 weeks 6 weeks were randomly divided into 4 groups with 10 mice in each group. They were normal control group, asthma model group, aminophylline treatment group and dexamethasone positive control group. The asthmatic model was established in the latter three groups. The airway reactivity of mice was measured by two factor factorial design. HE staining was used to observe the pathological morphology of mouse lung tissue, flow cytometry was used to detect the proportion of Tfh cells, and Western-blotting was used to detect the protein expression of Bcl-6. The levels of IL-4,IL-10 in alveolar lavage fluid and serum were measured by Elisa method. Results the airway resistance in asthmatic model group was significantly higher than that in normal group (P0.05). The airway smooth muscle was thickened and a large number of inflammatory cells infiltrated in the), HE staining section, and the inflammatory score was significantly higher than that in the normal group (P0.05). The percentage of Tfh cells in the spleen of mice was significantly higher than that of the control group, the content of Bcl-6 protein was significantly higher than that of the normal group, and the content of IL-4 in serum and alveolar lavage fluid was significantly higher than that in the normal control group. However, the level of IL-10 decreased significantly. The airway resistance in the aminophylline treatment group and the dexamethasone positive control group was significantly lower than that in the asthma model group (P0.05). The degree of inflammatory cell infiltration in the HE staining section of the mice was significantly lower than that in the asthmatic model group. The proportion of Tfh cells was significantly lower than that of asthma model group, the expression of Bcl-6 protein in lung tissue was significantly lower than that in asthmatic model group, the content of IL-4 in serum and alveolar lavage fluid was significantly lower than that in asthma model group, and the level of IL-10 was significantly higher than that in asthmatic model group. Conclusion Aminophylline can effectively improve airway inflammation and airway hyperresponsiveness in asthmatic mice by increasing the proportion of Tfh cells and the level of Bcl-6 protein, which may be related to the effect of aminophylline on the proportion of Tfh cells and the level of IL-4,IL-10.
【作者单位】： 河北省唐山市工人医院呼吸科;河北联合大学附属医院呼吸内科;
【分类号】：R562.25
，
本文编号：2410929
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