游离DNA完整性检测对良恶性胸腹腔积液鉴别诊断的价值研究
发布时间：2019-01-28 23:03
【摘要】：目的探讨游离DNA(cfDNA)的完整性(ALU247/ALU115)检测对良恶性胸腹腔积液鉴别诊断价值。方法采用实时荧光定量PCR方法,测定86份良恶性胸腹腔积液标本中ALU115和ALU247片段浓度并计算完整性;同时对标本中LDH、AFP、CEA、SF、SCC等传统指标进行检测。绘制受试者工作曲线(ROC曲线),根据曲线下面积评估各标记物诊断意义;运用统计学方法,与传统指标进行对比,评价cf DNA完整性在良恶性胸腹腔积液鉴别诊断中的应用价值。结果恶性组DNA完整性指数(ALU247/ALU115)显著高于良性组,差异具有统计学意义(P0.01),ROC曲线下面积为0.70。cfDNA浓度和完整性联合检测的灵敏度、特异度及准确度均显著高于其他各项指标单独或联合检测。该联合检测方式与cfDNA联合传统指标在鉴别诊断良恶性胸腹腔积液方面比较差异无统计学意义。结论 cfDNA完整性检测是良恶性胸腹腔积液鉴别诊断可能的良好靶标。ALU115、ALU247片段浓度和完整性(ALU247/ALU115)三项联合检测诊断价值更高。
[Abstract]:Objective to investigate the value of free DNA (cfDNA) integrity (ALU247/ALU115) in differential diagnosis of benign and malignant pleural and peritoneal effusion. Methods the concentrations of ALU115 and ALU247 fragments in 86 samples of benign and malignant pleural and peritoneal effusion were measured by real-time fluorescence quantitative PCR and the computational integrity was calculated. The traditional indexes such as LDH,AFP,CEA,SF,SCC in the samples were also detected. The subjects' operating curve (ROC curve) was drawn and the diagnostic significance of each marker was evaluated according to the area under the curve. To evaluate the value of cf DNA integrality in differential diagnosis of benign and malignant pleural and peritoneal effusion. Results the DNA integrity index (ALU247/ALU115) of malignant group was significantly higher than that of benign group, and the difference was statistically significant (P0.01), ROC curve area was the sensitivity of 0.70.cfDNA concentration and integrity combined detection. Specificity and accuracy were significantly higher than other indicators alone or combined detection. There was no significant difference in differential diagnosis of benign and malignant pleural and peritoneal effusion between this method and cfDNA combined with traditional indexes. Conclusion cfDNA integrity detection is a good target for differential diagnosis of benign and malignant pleural and peritoneal effusion, and the combined detection of ALU115,ALU247 fragment concentration and integrity (ALU247/ALU115) is more valuable.
【作者单位】： 南通大学附属医院医院感染管理办公室;南通大学附属医院医学检验中心;南通大学医学院免疫教研室;南通大学附属医院外科综合实验室;
【基金】：国家自然科学基金资助项目(编号:81672099) 江苏省科技项目(编号:BE2015654) 江苏省医学创新团队与领军人才项目(编号:LJ201133) 南通市社会发展基金项目(编号:HS2015002)
【分类号】：R561.3
，
本文编号：2417397
[Abstract]:Objective to investigate the value of free DNA (cfDNA) integrity (ALU247/ALU115) in differential diagnosis of benign and malignant pleural and peritoneal effusion. Methods the concentrations of ALU115 and ALU247 fragments in 86 samples of benign and malignant pleural and peritoneal effusion were measured by real-time fluorescence quantitative PCR and the computational integrity was calculated. The traditional indexes such as LDH,AFP,CEA,SF,SCC in the samples were also detected. The subjects' operating curve (ROC curve) was drawn and the diagnostic significance of each marker was evaluated according to the area under the curve. To evaluate the value of cf DNA integrality in differential diagnosis of benign and malignant pleural and peritoneal effusion. Results the DNA integrity index (ALU247/ALU115) of malignant group was significantly higher than that of benign group, and the difference was statistically significant (P0.01), ROC curve area was the sensitivity of 0.70.cfDNA concentration and integrity combined detection. Specificity and accuracy were significantly higher than other indicators alone or combined detection. There was no significant difference in differential diagnosis of benign and malignant pleural and peritoneal effusion between this method and cfDNA combined with traditional indexes. Conclusion cfDNA integrity detection is a good target for differential diagnosis of benign and malignant pleural and peritoneal effusion, and the combined detection of ALU115,ALU247 fragment concentration and integrity (ALU247/ALU115) is more valuable.
【作者单位】： 南通大学附属医院医院感染管理办公室;南通大学附属医院医学检验中心;南通大学医学院免疫教研室;南通大学附属医院外科综合实验室;
【基金】：国家自然科学基金资助项目(编号:81672099) 江苏省科技项目(编号:BE2015654) 江苏省医学创新团队与领军人才项目(编号:LJ201133) 南通市社会发展基金项目(编号:HS2015002)
【分类号】：R561.3
，
本文编号：2417397
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