IL-17F基因多态性与慢性阻塞性肺疾病相关性研究

发布时间：2019-02-26 20:36

【摘要】：目的探讨白细胞介素17F(IL-17F)基因位点rs763780多态性与慢性阻塞性肺疾病的相关性。 方法以2011年11月1日至2012年10月31日在广西医科大学第一附属医院门诊及体检中心就诊的患者作为研究对象,其中COPD患者125例,健康志愿者126例。根据吸烟史及肺通气功能结果将研究对象分为4组：吸烟COPD1～2级组(A组)42例,吸烟COPD3-4级组(B组)83例,吸烟健康组(C组)67例,不吸烟健康组(D)组)59例。收集他们的年龄、民族、吸烟史、Body Mass Index(BMI)、FEV1%、FEV1/FVC、FEF25、FEF50、 FEF75、MMEF75/25等指标,采集4ml外周静脉血提取基因组DNA。用Taqman探针标记荧光定量PCR法检测IL-17F第三外显子上rs763780位点的单核苷酸多态性(SNP),获取各组的基因型及等位基因分布情况。数据结果使用SPSS13.0软件进行统计学分析,探讨IL-17F rs763780基因多态性与COPD的相关性。 结果(1)IL-17F基因rs763780位点上各基因型的分布符合Hardy-Weinberg平衡。(2)A组CC、CT、TT基因型频率分别为：2.38%、30.95%、66.67%；B组分别为：1.20%、31.33%、67.47%；C组则分别为：0%、34.33%、65.67%；D组分别为：5.08%、28.81%、66.10%。四组基因型频率比较结果为x2=4.788,P=0.571。四组的C、T等位基因频率分别为：A组(17.86%、82.14%),B组(16.87%、83.13%),C组(17.16%、82.84%),D组(19.49%、80.51%),四组资料的C、T等位基因频率比较结果为X2=2.167,P=-0.538)。四组基因型及等位基因分布比较均无差别。(3)将COPD患者按照肺功能分为Ⅰ级、Ⅱ级、Ⅲ级、Ⅳ级。对IL-17F rs763780位点的三种基因型在COPD各级中的分布进行卡方检验,X2=14.34,P=0.07。意味着基因型在各级COPD中的分布无差别。(4)将吸烟指数与FEV1%进行相关性分析,发现两者呈密切负相关(r=-0.460,p=0.000)；年龄与FEV1%呈负相关(r=-0.139,P=0.032)。(5)使用Logistic回归分析排除年龄、BMI、家族史等混杂因素后探讨基因型对COPD发病的影响,发现三种基因型CC、CT、TT的回归系数检验(wald检验)P值分别为0.561、0.894、0.575,均大于0.05。说明三种基因型均非COPD发病的危险因素。(6)将三种基因型做为因变量建立广义logistic回归模型进行分析,发现吸烟及年龄影响IL-17F rs763780位点的基因突变。吸烟者更不易发生CC基因突变。(似然比P值为0.546,β=-0.103,P=0.021,OR=1.901)。年龄越大越不容易发生CT突变(似然比P值为0.859,p=-0.028、,P=0.029,OR=0.973) 结论IL-17F rs763780基因多态性与COPD易感性及疾病的严重程度无明显相关性,但年龄及吸烟影响基因突变。吸烟者不易发生CC基因突变,年龄越大者不易发生CT基因突变。
[Abstract]:Objective to investigate the association between interleukin 17F (IL-17F) gene rs763780 polymorphism and chronic obstructive pulmonary disease (COPD). Methods A total of 125 COPD patients and 126 healthy volunteers were selected from the outpatient and physical examination center of the first affiliated Hospital of Guangxi Medical University from November 1, 2011 to October 31, 2012. According to smoking history and pulmonary ventilation function, the subjects were divided into 4 groups: COPD1~2 group (group A, n = 42), COPD3-4 group (group B, n = 83), smoking healthy group (group C, n = 67) and non-smoking healthy group (D), n = 59). The age, nationality and smoking history of, Body Mass Index (BMI), FEV1%,FEV1/FVC,FEF25,FEF50, FEF75,MMEF75/25 were collected, and the genomic DNA. was extracted from the peripheral venous blood of 4ml. The single nucleotide polymorphism (SNP), at rs763780 site in exon 3 of IL-17F was detected by fluorescence quantitative PCR labeled with Taqman probe. The distribution of genotypes and alleles in each group was obtained. The data were analyzed by SPSS13.0 software to explore the correlation between IL-17F rs763780 gene polymorphism and COPD. Results (1) the distribution of genotypes at the rs763780 site of IL-17F gene was in accordance with Hardy-Weinberg equilibrium. (2) the frequencies of CC,CT,TT genotypes in group A were 2.38%, 30.95% and 66.67%, respectively. Group B: 1.20%, 31.33%, 67.47%, group C: 0%, 34.33%, 65.67%, group D: 5.08%, 28.81%, 66.10%, respectively. The results of comparison of genotype frequencies among the four groups were as follows: x2? 4.788, P0. 571. The allele frequencies of C and T in the four groups were as follows: group A (17.86%, 82.14%), B) (16.87%, 83.13%), C) (17.16%, 82.84%), D) (19.49%, 80.51%). The C and T allele frequencies of the four groups were X 2? 2.167, P < 0. 538 (P < 0. 538). There was no difference in genotype and allele distribution among the four groups. (3) patients with COPD were divided into grade 鈪，

本文编号：2431126