孟鲁司特对循环纤维细胞参与哮喘小鼠气道重塑的影响

发布时间：2019-03-04 08:26

【摘要】：目的:研究应用孟鲁司特在哮喘小鼠气道重塑中作用及对肺组织中循环纤维细胞(CF)的影响。方法:将30只小鼠随机分为对照组、哮喘组、孟鲁司特组,每组10只;HE染色观察支气管基底膜和平滑肌厚度改变;Masson染色观察胶原沉积;免疫组化观察肺组织中CF的表达及定位。各组基底膜厚度、平滑肌厚度与CF数目关系做直线相关性分析。结果:与对照组相比,哮喘组小鼠基底膜厚度、平滑肌厚度和胶原面积增厚(P0.01),支气管周围CF数量增加(P0.01)。孟鲁司特处理后,基底膜厚度(P0.01)、平滑肌厚度(P0.01)、胶原沉积面积(P0.01)及CF数量(P0.01)较哮喘组明显减少。基底膜厚度和平滑肌厚度与CF数目有相关性。结论:基底膜厚度和平滑肌厚度与CF数目成正相关,孟鲁司特能够通过抑制肺组织中CF增加,从而延缓气道重塑进程。
[Abstract]:Aim: to study the role of montelukast in airway remodeling and the effect of montelukast on circulating fibroblast (CF) in asthmatic mice. Methods: 30 mice were randomly divided into control group (n = 10), asthma group (n = 10) and montelukast group (n = 10), the thickness of bronchial basement membrane and smooth muscle were observed by HE staining, collagen deposition was observed by Masson staining. The expression and localization of CF in lung tissue were observed by immunohistochemistry. The relationship between basal membrane thickness, smooth muscle thickness and the number of CF was analyzed by linear correlation analysis. Results: compared with the control group, the thickness of basement membrane, the thickness of smooth muscle and the area of collagen in asthma group increased (P0.01), and the number of CF around bronchus increased (P0.01). After treatment with montelukast, the thickness of basement membrane (P0.01), the thickness of smooth muscle (P0.01), the area of collagen deposition (P0.01) and the number of CF (P0.01) were significantly lower than those in asthma group. The thickness of basement membrane and smooth muscle were correlated with the number of CF. Conclusion: the thickness of basement membrane and the thickness of smooth muscle are positively correlated with the number of CF. Montelukast can delay airway remodeling by inhibiting the increase of CF in lung tissue.
【作者单位】：武汉大学中南医院呼吸内科;
【分类号】：R562.25

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