染矽小鼠体内KL-6含量与肺纤维化关系

发布时间：2019-04-02 15:03

【摘要】：目的探讨染矽小鼠血液及支气管肺泡灌洗液(BALF)中涎液化糖链抗原-6(KL-6)含量与肺纤维化关系。方法雌雄各半的60只C57BL/6小鼠,随机分为染矽组和对照组,染矽组经气管滴入二氧化硅,对照组滴入生理盐水;各组小鼠于染硒后30、45、60、75、90 d分批处死,收集血液和BALF检测KL-6含量;肺组织切片行Masson胶原染色,显微镜评估肺纤维化程度。结果染矽组小鼠染硒45~90 d肺组织出现胶原沉积;与染矽45 d小鼠比较,染矽60、75、90 d小鼠肺纤维化评分值均显著增加(P0.01);染矽60、75、90 d小鼠血液KL-6含量明显高于对照组和染矽45 d小鼠(P0.01);染矽75、90 d小鼠BALF中KL-6含量明显高于对照组和染矽60 d小鼠(P0.01);小鼠血液及BALF中KL-6含量与纤维化评分值呈正相关(r=0.866、0.737,P0.01)。结论小鼠染矽45~90 d出现肺纤维化,血液及BALF中KL-6含量与肺纤维化程度呈正相关。
[Abstract]:Aim to investigate the relationship between the content of KL-6 in the blood and bronchoalveolar lavage fluid (BALF) of mice exposed to silica and pulmonary fibrosis. Methods Sixty male and female C57BL/6 mice were randomly divided into silicon-exposed group and control group. Silica was infused into the trachea of the silicon-exposed group and normal saline was injected into the control group. The mice in each group were killed at 30, 45, 60, 75, 90 days after selenium treatment, blood and BALF were collected to detect the content of KL-6, lung tissue sections were stained with Masson collagen, and the degree of pulmonary fibrosis was evaluated by microscope. Results Collagen deposition was observed in lung tissue of mice exposed to selenium for 45 d, and the score of pulmonary fibrosis was significantly increased at 60 d, 75 d and 90 d after exposure to Si (P0.01) compared with the mice exposed to Si for 45 d, 75 d and 90 d, respectively (P 0.01). The levels of KL-6 in blood of mice exposed to silicon for 60, 75 and 90 days were significantly higher than those of the control group and the mice exposed to silicon for 45 days (P0.01), and the content of KL-6 in the BALF of the mice exposed to silicon for 75 and 90 days was significantly higher than that of the control group and the mice exposed to silicon for 60 days (P0.01). There was a positive correlation between the content of KL-6 in blood and BALF and the score of fibrosis (r = 0.866, 0.737, P0.01). Conclusion Pulmonary fibrosis occurs in mice after exposure to silicon for 45 days. There is a positive correlation between the content of KL-6 in blood and BALF and the degree of pulmonary fibrosis.
【作者单位】：湖州师范学院医学院创新实验室;湖州师范学院微生物与免疫学研究所;
【基金】：浙江省公益技术应用研究计划实验动物项目(2012C37099)
【分类号】：R135.2

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