不同结核类型患者外周血IL-6与IL-35和IL-37及TNF-α水平的动态变化研究

发布时间：2019-04-26 01:28

【摘要】：目的探讨活动性肺结核(ATB)、潜伏结核感染(LTBI)患者外周血白介素-6(IL-6)、白介素-35(IL-35)、白介素-37(IL-37)、肿瘤坏死因子-α(TNF-α)水平动态变化及意义。方法选择医院2017年4月-8月收治的ATB患者43例,设为ATB组,43例LTBI患者,设为LTBI组,另选择同期健康体检者43名设为对照组,通过酶联免疫吸附法(ELISA)分别检测三组受试者血清IL-6、IL-35、IL-37、TNF-α水平,比较不同病情、有无胸水活动性肺结核、肺结核组治疗后患者血清指标,并通过Spearman相关分析活动性肺结核患者血清IL-6、IL-35、IL-37、TNF-α表达水平的相关性。结果 ATB组、LTBI组血清IL-6、IL-35、IL-37、TNF-α水平均显著高于对照组(P0.05),且ATB组上述指标均显著高于LTBI组(P0.05);伴空洞ATB患者血清IL-35、IL-37水平均显著高于非空洞患者(P0.05);伴胸水ATB患者血清IL-6、IL-35水平均明显著高于无胸水患者(P0.05);ATB患者治疗后血清IL-6、IL-35、IL-37、TNF-α水平均显著低于治疗前(P0.05);Spearman相关分析发现ATB患者血清IL-6水平与IL-35、IL-37分别正相关(P0.05),与TNF-α无关,IL-35与IL-37、TNF-α分别正相关(P0.05),IL-37与TNF-α正相关(P0.05)。结论 IL-6、IL-35、IL-37及TNF-α对结核感染状态有一定的鉴别判断作用,IL-35等细胞因子动态变化对ATB病情程度、疗效评估有重要意义。
[Abstract]:Objective to investigate the levels of interleukin-6 (IL-6), interleukin-35 (IL-35) and interleukin-37 (IL-37) in patients with active pulmonary tuberculosis (ATB),) with latent tuberculosis (LTBI). Dynamic changes of tumor necrosis factor-伪 (TNF- 伪) level and its significance. Methods from April to August 2017, 43 patients with ATB were divided into ATB group (n = 43), LTBI group (n = 43) and control group (n = 43). The serum levels of IL-6,IL-35,IL-37,TNF- 伪 were measured by enzyme-linked immunosorbent assay (ELISA) in the three groups. The serum indexes of patients with active pulmonary tuberculosis in pleural effusion and pulmonary tuberculosis group were compared between the three groups. Spearman correlation was used to analyze the correlation of serum IL-6,IL-35,IL-37,TNF- 伪 expression in patients with active pulmonary tuberculosis. Results the levels of serum IL-6,IL-35,IL-37,TNF- 伪 in ATB group and LTBI group were significantly higher than those in control group (P0.05), and the above indexes in ATB group were significantly higher than those in LTBI group (P0.05). The level of serum IL-35,IL-37 in patients with cavity ATB was significantly higher than that in patients without pleural effusion (P0.05), and the level of serum IL-6,IL-35 in patients with pleural effusion ATB was significantly higher than that in patients without pleural effusion (P0.05). The levels of serum IL-6,IL-35,IL-37,TNF- 伪 in patients with ATB after treatment were significantly lower than those before treatment (P0.05). Spearman correlation analysis showed that serum IL-6 levels in ATB patients were positively correlated with IL-35,IL-37 (P0.05), but not with TNF- 伪, while IL-35 and IL-37,TNF- 伪 were positively correlated (P0.05). There was a positive correlation between IL-37 and TNF- 伪 (P0.05). Conclusion IL-6,IL-35,IL-37 and TNF- 伪 can differentiate and judge the infection status of tuberculosis. The dynamic changes of IL-35 and other cytokines have important significance in evaluating the severity and curative effect of ATB.
【作者单位】：武汉市肺科医院呼吸内科;武汉市肺科医院结核内科;
【基金】：武汉市卫计委科研基金资助项目(WX171229)
【分类号】：R521

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