N-乙酰半胱氨酸对吸烟导致小鼠肺气肿及IL-12水平的影响

发布时间：2019-05-08 23:07

【摘要】：目的观察N-乙酰半胱氨酸(NAC)对烟草烟雾暴露导致的小鼠肺气肿及白介素-12(IL-12)水平的影响。方法健康雄性C57BL/6小鼠24只,按随机数字表法分为正常对照组、烟雾暴露组和NAC组。烟雾暴露组和NAC组小鼠给予香烟烟雾吸入共20周。NAC组小鼠在烟雾暴露16周时,予以加用NAC腹腔内注射给药,4周后处死小鼠,留取肺组织、血液和肺泡灌洗液(BALF)。使用HE染色法观察小鼠肺组织病理形态改变,计算单位面积内的平均肺泡内衬间隔以及肺泡数;使用酶联免疫吸附法(ELISA)检测小鼠BALF和血清中IL-12的浓度水平。结果与正常对照组相比,烟雾暴露组小鼠肺组织内炎症细胞浸润及肺气肿样变化明显,肺泡内衬间隔增加,肺泡数减少。同时,血清及BALF内IL-12水平较正常对照组明显增高,差异有统计学意义(P0.05);NAC组小鼠肺组织病理变化与烟雾暴露组相比明显减轻,差异有统计学意义(P0.05);血清和BALF内IL-12水平较烟雾暴露组显著降低,差异有统计学意义(P0.05)。结论 NAC能明显抑制烟雾暴露导致的肺气肿,降低炎症因子IL-12水平,提示NAC对烟雾暴露导致的小鼠肺部炎症反应及肺气肿可能有减轻作用。
[Abstract]:Aim to observe the effects of N-acetylcysteine (NAC) on emphysema and interleukin-12 (IL-12) levels in mice exposed to tobacco smoke. Methods 24 healthy male C57BL/6 mice were randomly divided into three groups: normal control group, smoke exposure group and NAC group. The mice in the smoke exposure group and the NAC group were given cigarette smoke inhalation for a total of 20 weeks, and the mice in the NAC group were injected intraperitoneally with NAC at 16 weeks after smoke exposure. After 4 weeks, the mice were killed and the lung tissue, blood and alveolar lavage fluid (BALF).) were retained. The pathological changes of mice lung tissue were observed by HE staining, the mean alveolar lining interval and alveolar number were calculated within the unit area, and the concentrations of BALF and IL-12 in serum were measured by enzyme-linked immunosorbent assay (ELISA). Results compared with the normal control group, the inflammatory cell infiltration and emphysema-like changes in the lung tissue of the smog-exposed mice were obvious, the alveolar lining interval increased and the number of alveoli decreased. At the same time, the level of IL-12 in serum and BALF was significantly higher than that in normal control group, the difference was statistically significant (P0.05). (P 0.05) the pathological changes of lung tissue in); NAC group were significantly reduced compared with smoke exposure group, the difference was statistically significant (P0.05). The levels of IL-12 in serum and BALF were significantly lower than those in smoke exposure group (P0.05). Conclusion NAC can significantly inhibit emphysema induced by smoke exposure and decrease the level of inflammatory factor IL-12, suggesting that NAC may have a protective effect on pulmonary inflammation and emphysema induced by smoke exposure in mice.
【作者单位】：安徽医科大学附属省立医院呼吸内科;
【基金】：安徽省科技厅计划项目(编号:1308085MH115)
【分类号】：R563.3

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