α-烯醇化酶在慢性阻塞性肺疾病合并肺腺癌患者肋间肌水平的研究
发布时间：2019-05-09 20:00
【摘要】：目的通过测定α-烯醇化酶及其mRNA在肺腺癌、慢性阻塞性肺疾病(COPD)合并肺腺癌患者肋间肌中的表达情况,探讨α-烯醇化酶在COPD中的调控情况。方法选取2012年4—10月就诊于昆明医科大学第一附属医院胸外科需行开胸肿瘤切除术的住院患者28例。根据术后肿瘤组织病理类型和中华医学会呼吸学分会慢性阻塞性肺疾病学组颁布的《慢性阻塞性肺疾病诊疗指南》(2008年修订版)中的诊断标准分为肺腺癌组12例和COPD合并肺腺癌组16例。采用实时定量荧光PCR(RT-PCR)测定α-烯醇化酶mRNA表达水平,采用ELISA法测定α-烯醇化酶蛋白水平。结果 COPD合并肺腺癌组α-烯醇化酶mRNA表达水平为(3.53±0.84),高于肺腺癌组的(2.36±0.63)(t=10.53,P0.01)。COPD合并肺腺癌组α-烯醇化酶蛋白水平为(1 175.51±219.16)ng/L,高于肺腺癌组的(1 047.29±120.83)ng/L(t=3.569,P0.01)。COPD合并肺腺癌患者肋间肌α-烯醇化酶mRNA表达水平与性别、年龄、第1秒用力呼气末容积占用力肺活量百分比(FEV1/FVC)、氧分压(PO2)、二氧化碳分压(PaCO2)无直线相关关系(r=0.670、0.545、0.498、0.658、0.420,P0.05),与第1秒用力呼气末容积占预计值百分比(FEV1/Pred)呈负相关(r=-0.713,P0.05)。COPD合并肺腺癌患者肋间肌α-烯醇化酶蛋白水平与性别、年龄、FEV1/FVC、PO2、PaCO2无直线相关关系(r=0.544、0.608、0.435、0.590、0.498,P0.05),与FEV1/Pred呈负相关(r=-0.819,P0.05)。结论 COPD可能通过调控α-烯醇化酶上游基因使肋间肌α-烯醇化酶mRNA表达水平及蛋白表达异常,从而影响患者肋间肌功能状态。
[Abstract]:Aim to determine the expression of 伪-enolase and its mRNA in intercostal muscles of patients with lung adenocarcinoma, chronic obstructive pulmonary disease (COPD) and lung adenocarcinoma, and to explore the regulation of 伪-enolase in COPD. Methods from April to October 2012, 28 inpatients who needed thoracotomy in the first affiliated Hospital of Kunming Medical University were selected. According to the pathological types of postoperative tumor tissue and the diagnostic criteria of chronic obstructive pulmonary disease (COPD) issued by the respiratory branch of Chinese Medical Association (revised 2008 edition), the patients were divided into two groups: lung adenocarcinoma group (n = 12) and chronic obstructive pulmonary disease (COPD) group (revised edition, 2008). There were 16 cases of COPD complicated with lung adenocarcinoma. The expression level of 伪-enolase mRNA was measured by real-time quantitative fluorescence PCR (RT-PCR), and the level of 伪-enolase protein was determined by ELISA method. Results the expression level of 伪-enolase mRNA in COPD with lung adenocarcinoma group was (3.53 卤0.84), which was higher than that in lung adenocarcinoma group (2.36 卤0.63) (t = 10.53, P < 0.01). The level of 伪-enolase protein in lung adenocarcinoma group was (1 175.51 卤219.16) ng/L, which was higher than that in lung adenocarcinoma group (1 047.29 卤120.83) ng/L (t = 3. 569, P < 0. 01). (P0.01). The expression level of 伪-enolase mRNA in intercostal muscle was correlated with sex, age, forced end expiratory volume to forced vital capacity (FEV1/FVC) and partial oxygen pressure (PO2) in the first second of forced expiratory volume in patients with COPD complicated with lung adenocarcinoma. There was no linear correlation between partial pressure of carbon dioxide (PaCO2) (r 鈮，
本文编号：2473044
[Abstract]:Aim to determine the expression of 伪-enolase and its mRNA in intercostal muscles of patients with lung adenocarcinoma, chronic obstructive pulmonary disease (COPD) and lung adenocarcinoma, and to explore the regulation of 伪-enolase in COPD. Methods from April to October 2012, 28 inpatients who needed thoracotomy in the first affiliated Hospital of Kunming Medical University were selected. According to the pathological types of postoperative tumor tissue and the diagnostic criteria of chronic obstructive pulmonary disease (COPD) issued by the respiratory branch of Chinese Medical Association (revised 2008 edition), the patients were divided into two groups: lung adenocarcinoma group (n = 12) and chronic obstructive pulmonary disease (COPD) group (revised edition, 2008). There were 16 cases of COPD complicated with lung adenocarcinoma. The expression level of 伪-enolase mRNA was measured by real-time quantitative fluorescence PCR (RT-PCR), and the level of 伪-enolase protein was determined by ELISA method. Results the expression level of 伪-enolase mRNA in COPD with lung adenocarcinoma group was (3.53 卤0.84), which was higher than that in lung adenocarcinoma group (2.36 卤0.63) (t = 10.53, P < 0.01). The level of 伪-enolase protein in lung adenocarcinoma group was (1 175.51 卤219.16) ng/L, which was higher than that in lung adenocarcinoma group (1 047.29 卤120.83) ng/L (t = 3. 569, P < 0. 01). (P0.01). The expression level of 伪-enolase mRNA in intercostal muscle was correlated with sex, age, forced end expiratory volume to forced vital capacity (FEV1/FVC) and partial oxygen pressure (PO2) in the first second of forced expiratory volume in patients with COPD complicated with lung adenocarcinoma. There was no linear correlation between partial pressure of carbon dioxide (PaCO2) (r 鈮，
本文编号：2473044
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