不同品系小鼠肺纤维化模型的比较研究

发布时间：2019-05-11 00:38

【摘要】：目的通过对博来霉素诱导3个不同品系小鼠肺纤维化程度的比较研究,寻找最适合研究肺纤维化的小鼠模型。方法选用C57BL/6、BALB/c以及KM 3种品系的小鼠,分别设为模型组和对照组。模型组向气管注射博来霉素(5 mg/kg),正常对照组注入等量的生理盐水。3个品系的小鼠分别于造模后的第7天、14天和28天处死8只,观察在不同的时间点肺系数、羟脯氨酸含量的变化以及通过Masson染色观察肺纤维化程度的改变。结果各模型组不同时间点的肺系数、羟脯氨酸含量和肺组织的病理学改变积分与对照组比较,差异有统计学意义(P0.05);C57BL/6模型组小鼠在不同时间点肺系数、羟脯氨酸含量和肺组织病理学改变积分与BALB/c和KM比较,差异有统计学意义(P0.05)。结论 C57BL/6小鼠对博来霉素反应敏感,肺纤维化明显,是3个品系小鼠中研究肺纤维化的最理想模型。
[Abstract]:Objective to find the most suitable mouse model for the study of pulmonary fibrosis by comparing the degree of pulmonary fibrosis induced by bleomycin in three different strains of mice. Methods C57BL / 6, BALB / c and KM mice were divided into two groups: model group and control group, which were divided into two groups: model group (model group) and control group (control group). The model group was injected with bleomycin (5 mg/kg) into the trachea, and the normal control group was injected with the same amount of normal saline. Eight mice of three strains were killed on the 7th, 14th and 28th day after modeling, and the lung coefficients were observed at different time points. The changes of hydroxyproline content and the degree of pulmonary fibrosis were observed by Masson staining. Results the lung coefficient, hydroxyproline content and pathological change score of lung tissue in each model group were significantly different from those in the control group at different time points (P 0.05). The lung coefficient, hydroxyproline content and pathological change score of lung tissue in C57BL/6 model group were significantly different from those in BALB/c and KM at different time points (P 0.05). Conclusion C57BL/6 mice are sensitive to bleomycin and have obvious pulmonary fibrosis, which is an ideal model for studying pulmonary fibrosis in three strains of mice.
【作者单位】：河北北方学院生物化学教研室;河北北方学院医学检验学院;
【基金】：张家口市科技局自然科学基金资助项目(No:-12110046D-1)
【分类号】：R563;R-332

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