间充质干细胞归巢到大鼠肺气肿组织分化为肺血管内皮细胞

发布时间：2019-05-31 14:50

【摘要】：背景:间充质干细胞具有独特的归巢、免疫调节及抗炎作用,经静脉注射移植后能归巢到损害的靶器官及组织发挥其功能,修复受损组织。目的:观察间充质干细胞移植后,肺气肿大鼠的肺组织病理改变。方法:将24只雌性Wistar大鼠随机分为实验组、对照组、健康对照组,前两组应用烟熏加气管内滴入猪胰弹性蛋白酶的方法制作肺气肿模型,造模成功后,实验组尾静脉注射BrdU标记骨髓间充质干细胞,对照组注射PBS。14 d后,检测观察大鼠肺组织病理学变化,检测肺泡灌洗液中肿瘤坏死因子α水平,肺泡壁细胞凋亡指数、抗CD34及抗Brd U免疫组织化学变化。结果与结论:①与健康对照组比较,对照组肿瘤坏死因子α水平、肺泡壁细胞凋亡指数升高(P0.01),抗Brdu及抗CD34相对面积减少(P0.01);与对照组比较,实验组肿瘤坏死因子α水平、肺泡壁细胞凋亡指数降低(P0.01),抗Brdu及抗CD34相对面积增加(P0.01);②肺组织病理学检查显示,对照组及实验组均呈现肺气肿样改变,但实验组轻于对照组;③结果表明,间充质干细胞能抑制实验性肺气肿的炎症反应及细胞凋亡,改善肺气肿病理学变化且骨髓间充质干细胞可分化为肺血管内皮细胞。
[Abstract]:Background: MSCs have unique homing, immunomodulatory and anti-inflammatory effects. After intravenous injection transplantation, MSCs can homing to damaged target organs and tissues to repair damaged tissues. Objective: to observe the pathological changes of lung tissue in emphysema rats after mesenchymal stem cell transplantation. Methods: 24 female Wistar rats were randomly divided into experimental group, control group and healthy control group. The first two groups were made emphysema model by fumigation and intratracheal infusion of pig pancreatic elastase. The experimental group was injected with BrdU labeled bone marrow mesenchymal stem cells, and the control group was injected with PBS.14 for d. The pathological changes of lung tissue, the level of tumor necrosis factor 伪 in alveolar lavage fluid and the apoptosis index of alveolar wall cells were detected. Immunohistochemical changes of anti-CD34 and anti-Brd U. Results and conclusion: 1 compared with the healthy control group, the level of tumor necrosis factor 伪 and the apoptosis index of alveolar wall cells in the control group were increased (P01), while the relative areas of anti-Brdu and anti-CD34 were decreased (P01). Compared with the control group, the level of tumor necrosis factor 伪 and the apoptosis index of alveolar wall cells in the experimental group were decreased (P 0.01), and the relative areas of anti-Brdu and anti-CD34 were increased (P 0.01). (2) the pathological examination of lung tissue showed that both the control group and the experimental group showed emphysema-like changes, but the experimental group was lighter than the control group. The results showed that MSCs could inhibit the inflammatory reaction and apoptosis of experimental emphysema, improve the pathological changes of emphysema, and bone marrow mesenchymal stem cells could differentiate into pulmonary vascular endothelial cells.
【作者单位】：河北省石家庄市第一医院胸外科;河北省石家庄市第一医院内分泌一科;
【基金】：石家庄市科技支撑计划项目(121461793)~~
【分类号】：R563.3

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