结核性胸腔积液的双向电泳蛋白质组学

发布时间：2019-06-04 19:02

【摘要】：目的:对结核性胸腔积液进行差异蛋白分析,尝试找出在结核性胸腔积液特征性表达的蛋白点,探索准确、快速、经济、简便的新型结核性胸膜炎实验诊断方法。方法:运用双向凝胶电泳技术对20例结核性胸膜炎、19例恶性胸腔积液和6例漏出液进行蛋白电泳分离,PDQuest8.0软件分析蛋白的等电点、分子量范围、灰度值等,最后进行凝胶图谱比较分析,得到差异蛋白点。结果:结核组与漏出组产生13个差异蛋白点,其中结核性胸腔积液较漏出液上调的蛋白点9个,下调的有4个;结核组与恶性组产生11个差异蛋白点,其中结核性胸腔积液较恶性上调的蛋白点5个,下调的有4个,仅在恶性胸腔积液表达的蛋白点2个。结论:双向电泳技术可获得分辨率和重复率较高的结核性、恶性和漏出性胸腔积液蛋白质凝胶图谱,三者存在差异蛋白点。
[Abstract]:Objective: to analyze the differential proteins of tuberculosis pleural effusions, to find out the protein spots characterized by the expression of tuberculosis pleural effusions, and to explore an accurate, rapid, economical and simple method for the experimental diagnosis of tuberculosis pleurisy. Methods: 20 cases of tuberculosis pleurisy, 19 cases of malignant pleural effusions and 6 cases of leakage were separated by two-dimensional gel electrophoresis. The isoelectric point, molecular weight range and gray value of the protein were analyzed by PDQuest8.0 software. Finally, the gel spectra were compared and analyzed, and the differential protein spots were obtained. Results: there were 13 different protein spots between tuberculosis group and leakage group, among which 9 protein spots were upregulated and 4 were down-regulated in tuberculosis pleural effusions. There were 11 different protein spots between tuberculosis group and malignant group, of which 5 were up-regulated, 4 were down-regulated and 2 were expressed in malignant pleural effusions. Conclusion: two-dimensional electrophoresis can obtain protein gel profiles of tuberculosis, malignant and leaky pleural effusions with high resolution and repetition rate, and there are differences in protein spots among the three.
【作者单位】：广州医科大学;广州市胸科医院呼吸疾病国家重点实验室;
【基金】：广州市科技计划项目(编号:2014Y2-00117);广州市科技计划课题(编号:155700012)
【分类号】：R521.7

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