高浓度氧气吸入对ARDS大鼠肺组织氧化应激的影响

发布时间：2019-06-12 05:30

【摘要】：目的探讨ARDS大鼠吸入高浓度氧气后肺组织氧化应激的变化。方法实验动物分为正常大鼠组和ARDS大鼠组,每组30只,每组再以吸入空气、60%氧气、100%氧气各分成三个亚组,每个亚组10只。72 h后留取动脉血行血氧分压(PO2)及丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、单胺氧化酶(MAO)测定;肺组织行HE染色观察Smith评分,RT-PCR检测Ⅱ型肺泡上皮细胞(AECⅡ)特异性肺泡表面活性蛋白C(SPC)和Ⅰ型肺泡上皮细胞(AECⅠ)特异性水通道蛋白5(AQP5)的mRNA表达。结果各组大鼠吸氧72 h后均存活。与正常大鼠空气亚组比较,正常大鼠100%氧气亚组Smith评分升高(3.77±1.20比0.83±0.26),P0.05;MDA升高(2.39±0.92比0.82±0.31),P0.05;SOD下降(51.99±21.73比86.44±26.38),P0.05;AQP5 mRNA下降(0.67±0.25比0.91±0.28),P0.05;ARDS大鼠空气亚组的Smith评分升高(11.65±3.82比0.83±0.26),P0.05;MDA升高(5.73±2.43比0.82±0.31),P0.05;SOD下降(45.71±14.68比86.44±26.38),P0.05;CAT下降(0.52±0.23比0.85±0.34),P0.05;AQP5 mRNA下降(0.28±0.12比0.91±0.28),P0.05;SPC mRNA下降(0.33±0.14比0.72±0.28),P0.05。ARDS组大鼠空气亚组的Smith评分(11.65±3.82比3.77±1.20),P0.05和AQP5 mRNA(0.28±0.12比0.67±0.25),P0.05;与正常大鼠100%氧气亚组差异显著。MAO在各组内和组间均差异无显著性(P0.05)。ARDS组大鼠动脉血氧分压100%氧气亚组高于60%氧气亚组(112.86±26.32比86.94±21.07),P0.05,60%氧气亚组高于空气亚组(86.94±21.07比65.05±15.19),P0.05。在ARDS组内与空气亚组比较,60%氧气亚组(0.43±0.18 VS 0.28±0.12),P0.05和100%氧气亚组(0.47±0.23比0.28±0.12),P0.05的AQP5 mRNA均有显著性升高;100%氧气亚组的Smith评分降低(7.88±2.53比11.65±3.82),P0.05,SPC mRNA升高(0.58±0.25比0.33±0.14),P0.05。结论高浓度氧气吸入和ARDS均可以影响氧自由基,氧自由基并不是高浓度氧的特征性产物,难以作为客观有效的氧毒性监测指标;高浓度氧气吸入对成年大鼠的肺部损害轻微且没有特异性;ARDS大鼠吸入100%氧气后没有出现叠加损害,反而比60%氧气更好地改善了肺部Smith评分和动脉血氧分压,增加了SPCmRNA和AQP5mRNA的表达,提示高浓度氧气对ARDS可能存在更为复杂的修复机制。
[Abstract]:Objective to investigate the changes of oxidative stress in lung tissue of ARDS rats after inhaling high concentration of oxygen. Methods the experimental animals were divided into normal rat group and ARDS rat group with 30 rats in each group. Each group was divided into three subgroups with inhaled air, 60% oxygen and 100% oxygen, 10 rats in each subgroup. 72 hours later, arterial blood was taken for partial pressure of oxygen (PO2) and malondialdehyde (MDA),) (SOD), catalase (CAT), monoamine oxidase (MAO). The Smith score was observed by HE staining. The mRNA expression of specific alveolar surface active protein C (SPC) and type I alveolar epithelial cell (AEC 鈪，

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