氯喹对哮喘小鼠气道高反应性的抑制作用

发布时间：2019-07-09 21:28

【摘要】：目的探讨氯喹对哮喘小鼠气道高反应性的作用及其可能机制。方法 Balb/c小鼠随机分为对照组、哮喘组、氯喹治疗组、地塞米松治疗组、氯喹+地塞米松治疗组,除对照组外,其余各组小鼠均给予OVA致敏和激发,建立哮喘小鼠模型,于每次激发前腹腔注射给药,对照组则于同一时间予等量PBS处理,末次激发后24 h内小鼠肺功能仪检测小鼠气道高反应性;检测支气管肺泡灌洗液(BALF)中细胞总数及分类计数;制作肺组织病理切片,HE染色后光镜下观察病理变化;ELISA检测支气管肺泡灌洗液中细胞因子IL-6、PGF2α水平。结果氯喹可明显降低哮喘小鼠气道高反应性(吸入25 mg/ml Ach时4.5±0.4 vs 6.1±0.9,P0.05,吸入50 mg/ml Ach时4.6±0.5 vs 8.2±1.0,P0.001);明显降低哮喘小鼠支气管肺泡灌洗液中炎症细胞数(P0.01);显著降低哮喘小鼠病理评分(P0.05);可一定程度降低哮喘小鼠BALF中PGF2α因子水平;与地塞米松合用可明显减轻哮喘小鼠支气管细支气管周围炎症(P0.05)、血管周围炎症(P0.01)、肺组织病理评分(P0.001),明显降低哮喘小鼠BALF中IL-6因子水平(P0.05)。结论氯喹可抑制哮喘小鼠气道高反应性;地塞米松与氯喹合用较单独用药效果更好,可能对中性粒细胞参与的哮喘有效。
[Abstract]:Objective to investigate the effect of chloroquine on airway hyperresponsiveness in asthma mice and its possible mechanism. Methods Balb/c mice were randomly divided into control group, asthma group, chloroquine treatment group, dexamethasone treatment group and chloroquine dexamethasone treatment group. Except the control group, all the mice in the other groups were sensitized and stimulated by OVA. The asthma mouse models were established and given intraabdominal injection before each challenge, while the control group was treated with the same amount of PBS at the same time. Within 24 hours after the last challenge, the airway hyperresponsiveness of the mice was detected by pulmonary function instrument. The total number and classified count of cells in bronchoalveolar lavage fluid (BALF) were detected, the pathological sections of lung tissue were made and the pathological changes were observed under light microscope after HE staining, and the level of cytokine IL-6,PGF2 伪 in bronchoalveolar lavage fluid was detected by ELISA. Results chloroquine significantly decreased airway hyperresponsiveness in asthma mice (4.5 卤0.4 vs 6.1 卤0.9 at 25 mg/ml Ach, P0.05, 4.6 卤0.5 vs 8.2 卤1.0 at 50 mg/ml Ach, P0.001), decreased the number of inflammatory cells in bronchoalveolar lavage fluid of asthma mice (P 0.01), significantly decreased the pathological score of asthma mice (P 0.05), and significantly decreased the number of inflammatory cells in bronchoalveolar lavage fluid of asthma mice (P 0.05), and significantly decreased the number of inflammatory cells in bronchoalveolar lavage fluid of asthma mice (P 0.05). The level of PGF2 伪 factor in BALF of asthma mice was decreased to a certain extent, and the level of IL-6 factor in BALF of asthma mice was significantly decreased by combination with dexamethasone (P 0.05), perivascular inflammation (P 0.01), pathological score of lung tissue (P0.001). Conclusion chloroquine can inhibit airway hyperresponsiveness in asthma mice, and dexamethasone combined with chloroquine is more effective than chloroquine alone, which may be effective in asthma involving neutrophils.
【作者单位】：重庆医科大学附属儿童医院呼吸病学实验室;
【基金】：国家自然科学基金(81070014)~~
【分类号】：R562.25

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