血小板反应蛋白1在纤维化模型大鼠肾组织中的表达

发布时间：2017-12-27 11:43

本文关键词：血小板反应蛋白1在纤维化模型大鼠肾组织中的表达　出处：《中国组织工程研究》2016年27期 　论文类型：期刊论文

【摘要】：背景:在实验性炎症性肾脏疾病模型中血小板反应蛋白1是转化生长因子β1重要的内源活化剂。转化生长因子β1是在许多不同的炎性疾病过程中,导致组织纤维化的主要细胞因子,特别是在肾脏疾病。目的:探讨血小板反应蛋白1在纤维化肾组织中的表达。方法:将健康雄性SD大鼠随机分为假手术组与模型组,模型组大鼠右侧输尿管进行结扎,制备大鼠肾纤维化模型。术后3周,每周取血、取尿,用ELISA与Bradford法检测血清肌酐、血尿素氮水平及尿蛋白含量;处死大鼠后,取结扎侧肾脏固定,用Western blot方法检测血管内皮生长因子、转化生长因子β1和血小板反应蛋白1蛋白的表达,苏木精-伊红检测术后肾组织病理结构改变情况。结果与结论:(1)造模后1周,模型组大鼠尿蛋白、血清肌酐与尿素氮水平显著高于假手术组(P0.05),3周后,各指标差异更显著(P0.01),表明肾功能损伤加重;(2)模型组转化生长因子β1蛋白与血小板反应蛋白1蛋白表达量明显高于假手术组,而血管内皮生长因子蛋白表达量显著低于假手术组;(3)苏木精-伊红染色结果显示,结扎肾小管后大鼠肾组织严重病变。(4)结果提示:血小板反应蛋白1蛋白在大鼠纤维化肾组织中表达水平显著升高,且其表达水平与血管内皮生长因子蛋白和转化生长因子β1蛋白密切相关,可能在肾纤维化中发挥重要作用。
[Abstract]:Background: in an experimental inflammatory renal disease model, platelet reactive protein 1 is an important endogenous activator of TGF - beta 1. Transforming growth factor beta 1 (TGF) is a major cytokine that leads to tissue fibrosis in many different inflammatory diseases, especially in kidney disease. Objective: To investigate the expression of platelet reactive protein 1 in the fibrotic renal tissue. Methods: the healthy male SD rats were randomly divided into the sham operation group and the model group. The right ureter was ligated in the model group, and the rat model of renal fibrosis was prepared. After 3 weeks, weekly blood, urine, blood urea nitrogen level and serum creatinine, blood urine protein content by ELISA and Bradford; rats were killed after taking the ligated kidney fixation, transforming growth factor beta 1 and anti platelet expression protein 1 protein by Western blot method for detection of vascular endothelial growth factor and, hematoxylin and eosin detection of postoperative renal pathological changes of structure. Results and conclusion: (1) 1 weeks after modeling, the rats in the model group of urine protein, serum creatinine and urea nitrogen levels were significantly higher than those in sham group (P0.05), after 3 weeks, the index more significant differences (P0.01), showed that the renal function injury; (2) the model group on expression of transforming growth factor beta 1 egg white and thrombospondin 1 protein was significantly higher than that in sham operation group, vascular endothelial growth factor protein expression was significantly lower than the sham operation group; (3) hematoxylin eosin staining showed that ligation of renal tubules after renal tissue of rats with severe disease. (4) the results suggest that the expression level of platelet reactive protein 1 is significantly elevated in rat fibrotic kidney tissue, and its expression level is closely related to vascular endothelial growth factor protein and transforming growth factor beta 1 protein, which may play an important role in renal fibrosis.
【作者单位】：石家庄市第一医院;
【基金】：2013年石家庄市科学技术研究与发展指导计划(131462423)~~
【分类号】：R692;R-332
【正文快照】： 0引言Introduction肾脏纤维化是不可逆转的、递进的慢性肾脏疾病,其特点是细胞外基质成分在肾小球和肾小管间质中持续积累[1]。肾脏纤维发生是一个动态的融合过程,几乎所有的肾脏细胞都参与其中,基质产生细胞的激活被认为是肾脏纤维发生的重要原因,包括成纤维细胞、肾小管上皮

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