吲哚胺2,3-二氧化酶在膀胱尿路上皮癌中表达及意义

发布时间：2018-01-04 05:36

本文关键词：吲哚胺2,3-二氧化酶在膀胱尿路上皮癌中表达及意义　出处：《中国现代医学杂志》2015年19期 　论文类型：期刊论文

【摘要】：目的探讨吲哚胺2,3-二氧化酶(IDO)在膀胱尿路上皮癌中的表达及意义。方法所有新鲜标本作荧光定量PCR,石蜡标本采用SP法进行免疫组化染色检测IDO的表达,并分析其与临床病理特征的关系。结果 IDO m RNA与IDO蛋白在正常膀胱组织中表达明显低于膀胱尿路上皮癌组织中表达,差异有统计学意义。在膀胱尿路上皮癌组织IDO表达强度与患者年龄、性别无关,与组织学分类、组织分级及UICC分期有相关性:浸润型与非浸润型之间差异有统计学意义(χ2=5.60,P=0.018);随着组织分级增高IDO阳性表达率增高,差异有统计学意义(χ2=20.27,P=0.000);随着UICC分期增高IDO阳性表达率增高,差异有统计学意义(χ2=12.08,P=0.007)。IDO m RNA表达与组织分级及UICC分期有相关,随着组织分级及UICC分期增高,表达水平增高,差异有统计学意义(t=2.73,P=0.011;t=4.96,P=0.000)。结论 IDO表达与膀胱尿路上皮癌预后不良相关,提示IDO可能成为膀胱尿路上皮癌预后的预测因子及治疗靶点。
[Abstract]:Objective to investigate the expression and significance of indomethylamine 2m3-dioxygenase (IDO) in bladder urothelial carcinoma. Methods all fresh specimens were detected by fluorescence quantitative PCR. Immunohistochemical staining was used to detect the expression of IDO in paraffin wax specimens. Results the expression of IDO m RNA and IDO protein in normal bladder tissue was significantly lower than that in bladder urothelial carcinoma tissue. The expression of IDO in bladder urothelial carcinoma was not related to age, sex, and histological classification. There was correlation between histological grading and UICC staging: there was significant difference between infiltrative type and non-invasive type (蠂 ~ 2 = 5.60). The positive expression rate of IDO increased with the increase of histological grading, and the difference was statistically significant (蠂 2 20. 27 P < 0. 000). With the increase of UICC stage, the positive expression rate of IDO increased, the difference was statistically significant (蠂 2 / 12. 08). The expression of P0. 007? Ido m RNA was correlated with histological grade and UICC stage. With the increase of tissue grade and UICC stage, the expression level increased. The difference was statistically significant (P < 0. 011). Conclusion the expression of IDO is associated with poor prognosis of bladder urothelial carcinoma, suggesting that IDO may be a predictor of prognosis and a therapeutic target for bladder urothelial carcinoma.
【作者单位】：云南省肿瘤医院;昆明医科大学第三附属医院;
【基金】：昆明医科大学-云南省科技厅联合专项(No:2010CD183)
【分类号】：R737.14
【正文快照】： 膀胱尿路上皮癌是泌尿系统最常见的恶性肿瘤,其发病率为泌尿系统肿瘤首位。膀胱尿路上皮癌具有局部易复发的生物学特性。免疫逃逸是肿瘤细胞的基本特征。肿瘤细胞可表达多种机体免疫系统能够识别并产生特异性免疫应答的肿瘤抗原,同时,各种肿瘤细胞可通过许多途径来逃避机体的

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