腺病毒介导的PDCD4基因过表达促进肾癌BTG1蛋白的表达和786-O细胞的凋亡

发布时间：2018-01-05 16:00

本文关键词：腺病毒介导的PDCD4基因过表达促进肾癌BTG1蛋白的表达和786-O细胞的凋亡　出处：《中国老年学杂志》2016年05期 　论文类型：期刊论文

【摘要】：目的探讨过表达抑癌基因PDCD4的腺病毒对肾透明细胞腺癌细胞株(786-O)的影响及肾脏注射后对肾癌的治疗效果。方法体外构建过表达抑癌基因PDCD4的腺病毒;培养786-O细胞株,转染腺病毒体外干预,观察细胞的死亡情况;将SD大鼠随机分为对照组和移植组,两组均在前肢腋部皮下注射786-O细胞株构建肾癌模型,造模14 d后,对照组瘤中注射0.1 ml D-Hanks,移植组则注射含1×10~(11)vp/m L的表达抑癌基因PDCD4的腺病毒的D-Hanks 0.1 ml。结果体外观察转染病毒后,786-O细胞的生长活力下降,死亡率升高;体内注射病毒后,可以观察到肾脏中抑癌基因PDCD4的上调和BTG1蛋白表达水平的升高。结论过表达抑癌基因PDCD4的腺病毒注射治疗肾癌具有可行性。
[Abstract]:Objective to investigate the effect of adenovirus overexpression of tumor suppressor gene PDCD4 on renal clear cell adenocarcinoma cell line 786-O). Methods Adenoviruses expressing tumor suppressor gene PDCD4 were constructed in vitro. 786-O cell line was cultured and transfected with adenovirus in vitro to observe the cell death. SD rats were randomly divided into two groups: control group and transplantation group. Both groups were injected with 786-O cell line subcutaneously into the armpit of forelimb to construct renal cell carcinoma model, and the model was made 14 days later. In the control group, 0.1 ml D-Hanks were injected into the tumor. The transplanted group was injected with D-Hanks 0.1 ml of adenovirus containing 1 脳 10 ~ (-1) v / m L PDCD4. Results after transfection of the virus in vitro, the expression of tumor suppressor gene PDCD4 was observed. The growth activity of 786-O cells decreased and the mortality increased. After the virus was injected into the body. The upregulation of tumor suppressor gene PDCD4 and the increase of BTG1 protein expression can be observed in kidney. Conclusion it is feasible to treat renal cell carcinoma with adenovirus injection with overexpression of tumor suppressor gene PDCD4.
【作者单位】：金华市人民医院泌尿外科;
【基金】：金华市科学技术局资助课题(No.2013-3-057)
【分类号】：R737.11
【正文快照】： 加深对肾癌的深层认知,从基因水平寻找突破口将是治疗肾癌有效的途径〔1,2〕。程序化凋亡因子(PDCD)4是一种抑癌基因,能够通过启动e IF4A、e IF4G达到抑制细胞生长的效果。癌组织中的PDCD4表达下降甚至缺失,提示我们上调PDCD4表达可能是启动癌细胞凋亡的分子开关〔3〕。腺病毒

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