C3a-C3aR在尿酸促进人肾小管上皮细胞MCP-1表达中的作用

发布时间：2018-01-05 17:15

  本文关键词：C3a-C3aR在尿酸促进人肾小管上皮细胞MCP-1表达中的作用　出处：《福建医科大学学报》2015年06期 　论文类型：期刊论文

  更多相关文章： 肾小管上皮细胞 单核趋化蛋白- 尿酸

【摘要】：目的探讨尿酸(UA)对人肾小管上皮细胞单核趋化蛋白-1(MCP-1)表达的影响及其机制。方法体外培养人肾皮质近曲小管上皮细胞(HK-2),以不同浓度UA分别作用不同时间,利用逆转录聚合酶链反应(RT-PCR)检测MCP-1的mRNA水平。在UA诱导下,通过针对补体C3的小干扰RNA(C3siRNA)转染下调HK-2细胞内源性C3的表达、小分子C3aR阻断剂SB290157阻断C3a-C3aR作用、外源性C3a刺激促进C3aR激活3种方式抑制或激活C3aR的信号通路,并通过实时荧光定量PCR(Q-PCR)检测其对于MCP-1mRNA表达的影响。结果 150μmol/L UA干预HK-2细胞12h可上调MCP-1mRNA转录水平;C3siRNA转染或C3aR阻断能够抑制被UA上调的MCP-1mRNA的表达;C3a与UA共同进行干预时,显著增强UA对MCP-1mRNA的诱导作用。结论 UA可上调HK-2细胞MCP-1mRNA的表达,其机制可能与UA激活C3或C3aR有关。
[Abstract]:Objective to investigate the serum uric acid (UA) on human renal tubular epithelial cells of monocyte chemoattractant protein -1 (MCP-1) expression and its influence mechanism. In HK-2 cell culture method in vitro (HK-2), with different concentrations of UA for different time respectively, using reverse transcriptase polymerase chain reaction (RT-PCR) to detect the level of mRNA MCP-1. Induced by UA, RNA by small interfering C3 (C3siRNA) to complement the down-regulation of HK-2 cell transfection and expression of endogenous C3, small molecule C3aR inhibitor SB290157 blocked C3a-C3aR effect of exogenous C3a stimulation C3aR signaling pathway activation in 3 ways to inhibit or activate C3aR, and real-time PCR (Q-PCR) detection and its effect on the expression of MCP-1mRNA. Results 150 mol/L UA intervention HK-2 cells 12h can upregulate the transcription level of MCP-1mRNA; C3siRNA or C3aR block can inhibit transfection upregulates the expression of UA MCP-1mRNA and C3a UA to intervene; The induction of MCP-1mRNA was significantly enhanced by UA. Conclusion UA can up regulate the expression of MCP-1mRNA in HK-2 cells, and the mechanism may be related to UA activation of C3 or C3aR.

【作者单位】： 福建医科大学附属第一医院肾内科;
【基金】：福建省卫生厅青年科研课题(2012-1-25)
【分类号】：R692
【正文快照】： (2015)06-0355-05慢性肾脏疾病中,趋化因子如单核趋化蛋白-1(monocyte chemotactic protein-1,MCP-1)在肾组织中的表达增加常常伴随着肾脏纤维化的进展[1],尿液中的MCP-1可以早期预测肾脏疾病[2]。补体C3片段C3a受体(compleneut 3areceptor,C3aR)是一个55kDa大小的蛋白质,主要

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