不同晶相的草酸钙晶体引起肾上皮细胞的毒性差异

发布时间：2018-01-09 06:36

本文关键词：不同晶相的草酸钙晶体引起肾上皮细胞的毒性差异　出处：《无机化学学报》2016年05期 　论文类型：期刊论文

【摘要】：采用扫描电子显微镜(SEM)、激光共聚焦显微镜(LSM)、流式细胞仪、电感耦合等离子体发射光谱仪(ICP)等方法比较研究了尺寸约1.2μm的一水草酸钙(COM)和二水草酸钙(COD)晶体对非洲绿猴肾上皮细胞(Vero细胞)的损伤差异。COM和COD均能引起Vero细胞的细胞活力和超氧化物歧化酶(SOD)活力降低、丙二醛(MDA)含量升高以及碘化丙啶(PI)红染细胞数目增多,且均呈现浓度依赖性。在相同晶体浓度时,COM的细胞毒性明显比COD强,引起细胞表面表达的粘附分子透明质酸(HA)比COD多,粘附量也显著增加。相比于COM晶体,Vero细胞更容易内吞COD晶体。COM与COD晶体对肾上皮细胞的毒性差异与晶面的离子密度以及细胞与晶体间的静电作用等因素密切相关。本文结果将有助于从分子水平上和细胞水平上阐释草酸钙结石的形成机理。
[Abstract]:Scanning electron microscopy (SEM), laser confocal microscopy (LSM) and flow cytometry (FCM) were used. The effects of calcium oxalate monohydrate and calcium oxalate dihydrate on the renal epithelial cells of African green monkeys were studied by ICP and ICP methods. Com and COD could decrease the cell viability and SOD activity of Vero cells. The content of malondialdehyde (MDA) and the number of red stained cells were increased in a concentration-dependent manner. The cytotoxicity of com was stronger than that of COD at the same crystal concentration. The expression of hyaluronic acid (HA) on the cell surface was more than that of COD, and the adhesion was significantly increased compared with that of COM crystal. The toxicity of COD crystals. Com and COD crystals to renal epithelial cells is more easily ingested by Vero cells. The difference is closely related to the ion density of crystal plane and electrostatic interaction between cells and crystals. It will be helpful to explain the mechanism of calcium oxalate stone formation at molecular and cellular level.
【作者单位】：暨南大学生物矿化与结石病防治研究所;
【基金】：国家自然科学基金(No.21371077)资助项目
【分类号】：R692.4
【正文快照】： 0引言肾结石是一种临床常见的多发病,肾中结石的主要组分为草酸钙(Ca Ox)[1],其中一水草酸钙(COM)的发生率约是二水草酸钙(COD)的2倍[2]。COD结石与高钙尿[3-4],低枸橼酸水平[5]和高p H值[6]有密切的联系,而COM结石经常出现在正常尿钙情况下[7]。晶体粘附是导致肾结石形成的重

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