siVEGF增敏吉西他滨对人膀胱癌小鼠移植瘤的治疗作用

发布时间：2018-01-25 16:08

  本文关键词： 膀胱肿瘤 基因治疗 细胞毒药物　出处：《中国老年学杂志》2017年15期 　论文类型：期刊论文

【摘要】：目的探讨血管内皮生长因子小干扰RNA(si VEGF)增敏吉西他滨(GEM)对人膀胱癌T24细胞株在小鼠膀胱成瘤情况的影响及其对瘤细胞生长的治疗作用。方法采用细胞移植法分别把经过处理(治疗组)和未经过处理(对照组)的T24细胞接种到小鼠膀胱内,然后用256排极速CT定期检测,并在28 d时处死动物称瘤重量,测定瘤体积,进行组织病理学和细胞学检查。结果治疗组和对照组的成瘤率分别为40%(6/15)和93%(14/15),治疗组瘤细胞生长速度明显低于对照组(P0.05)。两组肿瘤重量和体积比较差异有统计学意义(P0.05)。组织病理学和细胞学检查结果显示,治疗组移植瘤细胞增殖能力减弱,恶性度下降,瘤细胞生长转移风险降低,而对照组则未出现相应的变化。结论模拟人的小鼠膀胱癌原位模型更接近人体膀胱肿瘤的微环境和向膀胱腔内生长的特征,故更适合临床膀胱癌的治疗研究。推测靶向基因治疗联合细胞毒药物的化疗策略极有可能为提升目前临床膀胱癌的治疗水平和提高膀胱癌病人的生存率带来帮助。
[Abstract]:Objective to investigate the sensitizing effect of vascular endothelial growth factor (VEGF) small interfering RNA(si (VEGF) on gemcitabine (GEM). Effect of human bladder cancer cell line T24 on bladder tumorigenesis in mice and its therapeutic effect on tumor cell growth. Methods treated (treatment group) and untreated (control group) were treated by cell transplantation. T24 cells were inoculated into the bladder of mice. The animals were killed at 28 days to weigh the tumor and measure the tumor volume. Histopathological and cytological examinations were performed. Results the tumorigenesis rates in the treatment group and the control group were 40 / 15 and 93 / 14 / 15, respectively. The growth rate of tumor cells in the treatment group was significantly lower than that in the control group (P 0.05). There was a significant difference in tumor weight and volume between the two groups. The histopathological and cytological results showed that the tumor growth rate in the treatment group was significantly lower than that in the control group. In the treatment group, the proliferative ability of transplanted tumor cells decreased, the degree of malignancy decreased, and the risk of tumor cell growth and metastasis decreased. Conclusion the in-situ model of human bladder cancer in mice is closer to the microenvironment of human bladder tumor and the characteristics of bladder cavity growth. Therefore, this study is more suitable for the treatment of bladder cancer. It is speculated that the chemotherapeutic strategy of targeted gene therapy combined with cytotoxic drugs may help to improve the current treatment level of bladder cancer and improve the survival rate of bladder cancer patients. .
【作者单位】： 吉林大学第二医院泌尿外科;
【基金】：吉林省自然科学基金项目(No.20150101195JC)
【分类号】：R737.14
【正文快照】： 肿瘤微环境对肿瘤的发生发展有着至关重要的影响。研究膀胱癌的理想动物模型除应与人体膀胱癌在组织结构和生物学行为上相似外,肿瘤还应生长在膀胱腔内(原位生长)[1,2]。本实验选用小鼠,把经过血管内皮生长因子小干扰RNA(si VEGF)表达质粒转染并在其内加进细胞毒药物吉西他滨(，

本文编号：1463150