槲皮素对人前列腺癌PC-3裸鼠移植瘤的实验性疗效

发布时间：2018-01-26 02:23

本文关键词： 槲皮素激素非依赖性前列腺癌 PC-3细胞裸鼠移植瘤　出处：《南京中医药大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：目前临床上对前列腺癌尤其进展到激素非依赖性前列腺癌尚无有效治疗方法,而药物抗肿瘤尤其是天然植物在国内外已成为肿瘤治疗研究的热点。近年来很多国内外研究发现槲皮素这种天然植物提取物对很多肿瘤细胞均有抑制生长和抑制侵袭作用。本实验旨在通过槲皮素作用于激素非依赖性前列腺癌PC-3荷瘤鼠模型,观察槲皮素对人前列腺癌PC-3裸鼠异种移植瘤的生长抑制作用效果,在此基础上,探讨槲皮素治疗人前列腺癌PC-3细胞的作用和机制,为槲皮素治疗激素非依赖性前列腺癌提供理论和实验依据。方法:理论研究部分首先叙述中医对前列腺癌的研究进展,其次了解中西医治疗激素非依赖性前列腺癌的现状,最后对槲皮素抗肿瘤作用机制作一综述。实验研究部分首先体外培养人前列腺癌PC-3细胞,再制备小鼠激素非依赖性前列腺癌模型,槲皮素分组干预荷瘤鼠,最后测定不同组别小鼠移植瘤体积及重量；了解槲皮素对人前列腺癌PC-3裸鼠异种移植瘤的生长抑制作用效果,探讨槲皮素治疗激素非依赖性前列腺癌的具体机制。结果：体内裸鼠实验表明,槲皮素对激素非依赖性前列腺癌具有实验治疗作用,并具有剂量依赖性,槲皮素低、中、高剂量组对人前列腺癌PC-3裸鼠移植瘤的相对肿瘤增殖率(T/C)分别为90.96%,72.46%,68.71%；抑瘤率达8.32%,24.80%,30.60%。结论：槲皮素对人前列腺癌PC-3细胞裸鼠移植瘤的生长具有较显著的抑制作用(P0.01),并具有剂量依赖性,表明槲皮素对激素非依赖性前列腺癌具有实验治疗作用。
[Abstract]:Objective: at present, there is no effective treatment for prostate cancer, especially for hormone independent prostate cancer. In recent years, many researches at home and abroad have found that quercetin, a natural plant extract, has inhibited the growth and inhibition of many tumor cells. The aim of this study was to investigate the effects of quercetin on steroid-independent prostate cancer (PC-3) bearing mice. To observe the inhibitory effect of quercetin on the growth of xenograft tumor of human prostate cancer (PC-3) in nude mice, and to explore the effect and mechanism of quercetin on human prostate cancer PC-3 cells. To provide theoretical and experimental basis for quercetin in the treatment of hormone-independent prostate cancer. Methods: in the part of theoretical study, the research progress of traditional Chinese medicine (TCM) on prostate cancer and the current situation of hormone independent prostate cancer treated by traditional Chinese medicine (TCM) and western medicine (WM) were described. Finally, the anti-tumor mechanism of quercetin was reviewed. Firstly, human prostate cancer PC-3 cells were cultured in vitro, and then mouse steroid independent prostate cancer model was established. Quercetin was used to treat tumor bearing mice. The volume and weight of transplanted tumor were measured in different groups of mice. To investigate the effect of quercetin on the growth inhibition of xenograft tumor of human prostate cancer (PC-3) in nude mice, and to explore the mechanism of quercetin in the treatment of hormone independent prostate cancer. Results: in vivo experiments in nude mice showed that quercetin had an experimental therapeutic effect on hormone independent prostate cancer in a dose-dependent manner, and quercetin was low and moderate. The relative tumor proliferation rate (T / C) of the high dose group to transplanted human prostate cancer PC-3 nude mice was 90.96 / 72.46 / 68.71, respectively. The tumor inhibition rate was 8.32% and 24.80%, 30.60%. Conclusion: quercetin can inhibit the growth of human prostate cancer PC-3 cells in nude mice in a dose-dependent manner. It is suggested that quercetin has an experimental therapeutic effect on hormone-independent prostate cancer.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.25

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