人膀胱癌5637细胞中miR-21表达及其对细胞增殖和侵袭的影响

发布时间：2018-01-26 11:33

本文关键词： 膀胱肿瘤微小RNA- 细胞增殖细胞侵袭　出处：《山东医药》2016年37期 　论文类型：期刊论文

【摘要】：目的观察miR-21在人膀胱癌5637细胞中的表达变化,探讨其对细胞增殖和侵袭的影响。方法用RT-PCR技术检测膀胱癌5637细胞和人正常膀胱上皮细胞中的miR-21表达。将培养好的5637细胞随机分为转染组、阴性对照组、空白对照组,前两组分别转染AS-miR-21(沉默miR-21)、无效寡核苷酸序列,空白对照组不做任何处理;分别于基因沉默后24、48、72 h采用CCK-8法测算细胞增殖指数,基因沉默后24 h用Transwell侵袭实验检测细胞侵袭能力。结果与正常膀胱上皮细胞相比,人膀胱癌5637细胞中miR-21表达上调;沉默miR-21后,转染组细胞增殖指数、侵袭能力低于阴性对照组、空白对照组(P均0.05)。结论 miR-21在人膀胱癌5637细胞中高表达,沉默miR-21基因后膀胱癌细胞的增殖能力和侵袭性降低。
[Abstract]:Objective to observe the expression of miR-21 in human bladder cancer cell line 5637. Methods RT-PCR technique was used to detect the expression of miR-21 in bladder cancer 5637 cells and human normal bladder epithelial cells. The machine was divided into transfection group. Negative control group, blank control group, the first two groups were transfected with AS-miR-21 (silencing miR-21), invalid oligonucleotide sequence, blank control group did not do any treatment; Cell proliferation index was measured by CCK-8 method at 24 ~ 4872 h after gene silencing. 24 h after gene silencing, Transwell invasion assay was used to detect the invasion ability of the cells. Results compared with the normal bladder epithelial cells, the expression of miR-21 was up-regulated in human bladder cancer 5637 cells. After silencing miR-21, the cell proliferation index and invasiveness of transfected group were lower than those of negative control group. Conclusion miR-21 is highly expressed in human bladder cancer 5637 cells, and the proliferation and invasiveness of bladder cancer cells are decreased after miR-21 gene silencing.
【作者单位】：郑州大学第一附属医院;新乡医学院第一附属医院;
【基金】：国家自然科学基金面上项目(81272823) 河南省教育厅科技创新团队支持计划(14IRTSTHN020)
【分类号】：R737.14
【正文快照】： microRNA是一组长度为18~24个核苷酸的内源性非编码单链小分子RNA,可以与靶mRNA特异性结合,导致靶mRNA降解或翻译抑制,从而导致转录后的基因沉默。因此,mircoRNA通过调节下游靶基因的表达从而参与细胞增殖、凋亡、侵袭等活动。microRNA与多种病理生理过程有关,膀胱癌组织中mic

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