外周血白细胞线粒体DNA拷贝数与前列腺癌发病风险和预后的相关性研究

发布时间：2018-01-27 04:13

本文关键词： 前列腺癌线粒体DNA 发病风险预后　出处：《中南大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：探索外周血白细胞的线粒体DNA (mtDNA)拷贝数与前列腺癌发病风险及预后的相关性。方法：采用实时荧光定量PCR法检测196例前列腺癌患者和196例年龄配对正常对照的外周血白细胞mtDNA拷贝数,分析mtDNA拷贝数与前列腺癌发病风险的关系；随访其中89例前列腺癌患者,观察两个预后指标即疾病无进展生存时间、总生存时间,分析mtDNA拷贝数与肿瘤预后的关系。结果：前列腺癌患者的外周血mtDNA拷贝数较正常对照的显著升高(中位数0.91和0.82；P0.001)。mtDNA拷贝数与人群的前列腺癌发病风险显著相关,且两者呈剂量反应关系(高四分位数vs低四分位数：OR=2.52，95%CI：1.35-4.70；Ptrend=0.011)。前列腺癌患者AJCC分期、Gleason评分越高,mtDNA拷贝数越高(P=0.002和P=0.012)。Cox单因素和多因素回归模型提示mtDNA拷贝数是前列腺癌患者疾病进展和死亡预后的独立危险因素。Kaplan-Meier生存分析显示,mtDNA高拷贝数的前列腺癌患者与低拷贝数的相比,疾病无进展生存时间、总生存时间要短(P=0.007和P0.001)。结论：外周血mtDNA拷贝数与前列腺癌发病风险显著相关。在前列腺癌患者中,增高的mtDNA拷贝数与肿瘤的不良预后有关。量化外周血mtDNA拷贝数可能对前列腺癌发病风险评估和预后判断有重要意义。
[Abstract]:Objective: to investigate the correlation between the mitochondrial DNA mtDNA copy number in peripheral blood leukocytes and the risk and prognosis of prostate cancer. Methods: the mtDNA copies of peripheral white blood leukocytes in 196 patients with prostate cancer and 196 age-matched normal controls were detected by real-time fluorescence quantitative PCR. To analyze the relationship between mtDNA copy number and the risk of prostate cancer. 89 patients with prostate cancer were followed up. Two prognostic indexes were observed: survival time without progression and total survival time. The relationship between mtDNA copy number and tumor prognosis was analyzed. Results: the number of mtDNA copies in peripheral blood of prostate cancer patients was significantly higher than that of normal controls (median 0.91 and 0.82respectively). The copy number of P0.001mtDNA was significantly correlated with the risk of prostate cancer in the population, and there was a dose-response relationship between them (high quartile vs low quartile: OR2.52). 95 CI: 1.35-4.70; AJCC staging and Gleason score were higher in patients with prostate cancer. The higher the copy number of mtDNA is, the higher P0. 002 and P0. 012 are. Cox univariate and multivariate regression models suggest that mtDNA copy number is an independent risk factor for disease progression and death prognosis in patients with prostate cancer. Kaplan-Meier survival analysis showed. Compared with low copy number prostate cancer patients with high copy number of mtDNA had no progressive survival time and the total survival time was shorter than that of P0. 007 and P0. 001. Conclusion: the number of mtDNA copies in peripheral blood is significantly associated with the risk of prostate cancer. The increased mtDNA copy number is related to the poor prognosis of the tumor. Quantifying the peripheral blood mtDNA copy number may play an important role in the risk assessment and prognosis of prostate cancer.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.25

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