冬凌草甲素对膀胱癌T24细胞增殖的抑制作用

发布时间：2018-01-28 13:29

本文关键词： 冬凌草甲素 T细胞吸光度抑制凋亡　出处：《中国当代医药》2013年14期 　论文类型：期刊论文

【摘要】：目的探讨不同浓度的冬凌草甲素(ORI)在不同时间点对膀胱癌T24细胞增殖的抑制作用,为临床决策提供参考依据。方法将冬凌草甲素作用于T24细胞,利用酶标仪在不同时间点(λ=570nm)测定不同浓度的冬凌草甲素吸光度,同样方式用MTT法检测细胞的抑制率,流式细胞仪测定细胞凋亡率,其数值均以均数±标准差表示,每组结果由两位研究者重复测量2次校对。结果随着培养时间的延长及浓度的增加,T24细胞的吸光度数值呈明显下降趋势,差异有统计学意义(P0.05)。浓度为8μmol/L时抑制率递增,16μmol/L时抑制率已接近50%,而32μmol/L时抑制率最高可达70%以上,变化显著,差异有统计学意义(P0.05)。随时间、浓度梯度的变化,T24细胞凋亡率不断增加,最高可达60%以上,差异有统计学意义(P0.05)。证明冬凌草甲素对膀胱癌T24细胞增殖的抑制具有明显的浓度-效应与时间-效应的关系。结论冬凌草甲素可诱导膀胱癌T24细胞凋亡,有明显的抗肿瘤作用。
[Abstract]:Objective to investigate the inhibitory effects of oridonin (ORI) at different concentrations on the proliferation of bladder cancer cell line T24 at different time points. Methods the T24 cells were treated with oridonin and the absorbance of oridonin at different concentrations was determined by enzyme labeling instrument at different time points (位 ~ (70) nm). In the same way, the inhibition rate of cells was detected by MTT method, and the apoptosis rate was measured by flow cytometry, which was expressed as mean 卤standard deviation. The results of each group were measured twice by two researchers. The results showed that the absorbance of T24 cells decreased with the increase of culture time and concentration. The difference was statistically significant (P 0.05). When the concentration of 8 渭 mol/L was 8 渭 mol/L, the inhibition rate increased by 16 渭 mol/L, and the inhibition rate was close to 50%. The highest inhibition rate of 32 渭 mol/L was more than 70%, the difference was significant (P 0.05). The apoptosis rate of T24 cells increased with the change of concentration gradient. The maximum can be more than 60%. The difference was statistically significant (P0.05). Conclusion oridonin can induce apoptosis of T24 cells. It has obvious anti-tumor effect.
【作者单位】：山西医科大学;山西医学科学院(山西大医院)泌尿外科;
【分类号】：R285.5;R737.14
【正文快照】： 冬凌草又称为冰凌草、雪花草,因形似蝶状冰凌片而得名,在冬凌草植被中提炼出的一种二萜类抗肿瘤天然有机化合物成分为冬凌草甲素(ORI)。近年来有大量文献曾研究冬凌草具有抗肿瘤作用[1-2],常用于食管癌、胃癌的治疗,其无明显毒副作用,效果较好[3]。临床研究中曾报道,冬凌草有

【参考文献】