适合治疗给药的大鼠抗肾小球基底膜肾炎模型的建立与应用

发布时间：2018-02-02 06:17

本文关键词： 抗肾小球基底膜肾炎固肾号尿蛋白免疫荧光抗血清效价　出处：《中草药》2017年17期 　论文类型：期刊论文

【摘要】：目的建立适合治疗给药的大鼠抗肾小球基底膜(GBM)肾炎模型。方法采用家兔多点sc大鼠肾小球基底膜与弗氏佐剂乳化物的方法进行免疫,两周1次,共5次,制备抗血清;分次给大鼠iv抗血清,检测血清循环免疫复合物(CIC)水平、肾小球基底膜免疫荧光强度、肾脏及肾小球基底膜病变,确定适合治疗给药的抗血清效价、免疫剂量、方式及时间。以固肾2号为治疗药物,验证模型的可行性。结果使用效价为1∶4的兔抗大鼠GBM血清4 m L,分3 d 4次iv免疫,免疫第6、8、10天,均能形成大鼠抗肾小球基底膜肾炎,免疫第6天,病变相对较轻,免疫第8天及第10天,病变基本稳定。验证实验结果显示,连续给药30 d,固肾2号显著降低模型大鼠24 h尿蛋白排出,减轻肾脏病变,对大鼠抗肾小球基底膜肾炎有明显治疗作用。结论使用效价为1∶4的兔抗大鼠GBM血清4 m L,分3 d 4次iv免疫,免疫后8 d,能形成适合治疗给药的大鼠抗肾小球基底膜肾炎模型。
[Abstract]:Objective to establish a rat model of anti-glomerular basement membrane glomerulonephritis (GBM) which is suitable for treatment. Methods the rat glomerular basement membrane was immunized with Freund's adjuvant emulsifier once a fortnight by the method of rabbit multi-point sc rat glomerular basement membrane and Freund's adjuvant emulsification. Antiserum was prepared for 5 times. The serum level of circulating immune complex (CICC), the immunofluorescence intensity of glomerular basement membrane and the pathological changes of renal and glomerular basement membrane were determined to determine the titer of antiserum suitable for treatment. The dose, mode and time of immunization. The feasibility of the model was verified by using Gushen 2 as the therapeutic drug. Results 4 ml of rabbit anti-rat GBM serum with titer of 1: 4 was used and immunized for 3 days and 4 times iv. On day 6 and day 10, all rats developed anti-glomerular basement membrane glomerulonephritis. On the 6th day of immunization, the lesion was relatively mild, and on the 8th and 10th day of immunization, the lesion was basically stable. The experimental results showed that the lesion was basically stable. After 30 days of continuous administration, Gushen No. 2 significantly decreased the excretion of urinary protein and alleviated the renal lesion in the model rats. Conclusion Rabbit anti-rat GBM serum with titer of 1: 4 was used for 4 mL, 3 days and 4 times iv immunization, 8 days after immunization. The model of anti-glomerular basement membrane glomerulonephritis was established in rats suitable for treatment.
【作者单位】：贵州省人口和计划生育科学研究技术指导所;天津药物研究院新药评价有限公司;
【分类号】：R-332;R692
【正文快照】： 抗肾小球基底膜肾炎是一种常见的,具有特征性病因的慢性肾小球肾炎,其病变特点是肾小球内有大量新月体形成、免疫球蛋白(Ig G)沿肾小球基底膜(GBM)线性沉积、血清抗GBM抗体增多[1],中草药Chinese Traditional and Herbal Drugs第48卷第17期2017年9月预后不良,易引起慢性肾衰竭

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