硫化氢对1型糖尿病大鼠肾诱导型一氧化氮合酶的影响

发布时间：2018-02-09 04:27

本文关键词： 硫化氢糖尿病肾诱导型一氧化氮合酶一氧化氮大鼠　出处：《中南大学学报(医学版)》2017年04期 　论文类型：期刊论文

【摘要】：目的:观察硫化氢(hydrogen sulfide,H_2S)对1型糖尿病大鼠肾诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)的影响。方法:将32只雄性SD大鼠随机分为4组(n=8):正常对照(NC)组、糖尿病(DM)组、糖尿病治疗(NaHS+DM)组和NaHS对照(NaHS)组。采用腹腔注射55 mg/kg链脲佐菌素复制1型糖尿病大鼠模型。造模成功后,NaHS+DM组和NaHS组大鼠腹腔注射56μmol/kg NaHS溶液进行干预治疗。8周后,分别测定各组大鼠血清和肾组织中总一氧化氮合酶(total nitric oxide synthase,T-NOS)、iNOS活性和一氧化氮(NO)水平;测定肾组织中谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性;采用透射电镜观察肾皮质超微结构变化;Western印迹检测肾组织iNOS蛋白表达。结果:与NC组相比,NaHS组各项指标间差异均无统计学意义(P0.05),而DM组大鼠血清和肾组织中T-NOS,iNOS活性和NO水平均显著增高(P0.01);肾组织GSH-Px活性明显下降(P0.01)。电镜观察显示DM组大鼠肾小球基底膜增厚、系膜基质增多,足突融合;iNOS蛋白表达明显升高。与DM组相比,NaHS+DM组血清和肾T-NOS,iNOS活性和NO水平明显下降(P0.01),GSH-Px活性明显升高(P0.01),肾超微结构损伤明显减轻,iNOS蛋白表达显著降低(P0.01)。结论:H2S对1型糖尿病大鼠肾损伤具有保护作用,其机制可能与下调iNOS活性和蛋白表达,降低肾NO生成相关。
[Abstract]:Objective: to observe the effect of H2S) on inducible nitric oxide synthase (iNOS) in kidney of type 1 diabetic rats. Methods: 32 male SD rats were randomly divided into 4 groups: normal control group and diabetic group. The model of type 1 diabetic rats was induced by intraperitoneal injection of 55 mg/kg streptozotocin. The rats in the NaHS DM group and NaHS group were treated by intraperitoneal injection of 56 渭 mol/kg NaHS solution for 8 weeks after the successful establishment of the model. The activity of total nitric oxide synthase total nitric oxide synthase T-NOSn iNOS and the activity of glutathione peroxidase peroxidase (GSH-Px) were measured in serum and renal tissues of rats in each group, and the activities of glutathione peroxidase peroxidase (GSH-Px) in renal tissue were measured. The ultrastructural changes of renal cortex were observed by transmission electron microscopy and the expression of iNOS protein in renal tissue was detected by Western blot. Results: compared with NC group, there was no significant difference in the expression of T-NOSiNOS in serum and renal tissue in DM group. The activity of GSH-Px in renal tissue decreased significantly (P 0.01). Electron microscope observation showed that glomerular basement membrane thickened in DM group. Mesangial matrix increased, Compared with DM group, the activity of T-NOSiNOS and the level of no in serum and kidney of NaHS DM group were significantly decreased. GSH-Px activity of P0.01 was significantly increased, and the expression of iNOS protein was significantly reduced in NHS DM group. Renal injury in type 1 diabetic rats has protective effect. The mechanism may be related to down-regulation of iNOS activity and protein expression, and decrease of no production in kidney.
【作者单位】：蚌埠医学院生理学教研室;蚌埠医学院生物科学系;蚌埠医学院机能实验中心;
【基金】：国家自然科学基金(81000074) 安徽省高校自然科学研究项目(KJ2015B006by,KJ2017A216,KJ2017A210) 蚌埠医学院科研项目(BYKY1621ZD) 国家级大学生创新创业训练项目(201510367007)~~
【分类号】：R587.2;R692.9

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