新疆地区维吾尔族人群Klotho基因多态性及血脂成分、电解质与草酸钙肾结石关系的研究

发布时间：2018-02-23 13:10

本文关键词： Klotho基因草酸钙结石血电解质血脂　出处：《安徽医科大学学报》2016年05期 　论文类型：期刊论文

【摘要】：目的探讨Klotho基因多态性及血脂、电解质与草酸钙结石的相关性。方法采用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)方法检测128例草酸钙结石患者(病例组)和94例健康志愿者(对照组)Klotho基因rs3752472、rs650439和rs1207568位点多态性,并分析病例组与对照组中基因型分布差异以及病例组中不同基因型携带者的电解质、血脂水平差异。结果 Klotho基因SNP rs3752472多态性,其两组基因型分布差异有统计学意义(OR=3.707,95%CI:1.861~7.384;χ~2=14.912,P0.05),病例组中C/C与C/T+T/T基因型携带者相比较,钙磷水平差异有统计学意义(P0.05),血镁、血钠水平差异无统计学意义;低密度脂蛋白胆固醇(LDL-C)水平差异有统计学意义;三酰甘油(TG)、总胆固醇(TC)以及高密度脂蛋白胆固醇(HDL-C)水平比较差异无统计学意义;其余两组病例组与对照组相比较基因型分布差异无统计学意义,病例组内不同基因型携带者电解质和血脂比较差异无统计学意义。结论 Klotho基因SNP rs3752472多态性,是与新疆地区维吾尔族人群草酸钙结石发病风险相关的;在病例组中,C/C基因型者的血钙、血磷及LDL-C均高于CT+TT者;Klotho基因rs3752472多态性位点可能通过调节钙磷代谢、LDL-C水平来影响草酸钙结石的成石。
[Abstract]:Objective to investigate the polymorphism of Klotho gene and blood lipids. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the Klotho base in 128 patients with calcium oxalate stone (case group) and 94 healthy volunteers (control group). Because of rs3752472 rs650439 and rs1207568 polymorphism, The differences of genotype distribution and electrolyte and blood lipid levels in patients with different genotypes were analyzed. Results the polymorphism of SNP rs3752472 in Klotho gene was analyzed. There were significant differences in genotype distribution between the two groups (P = 3.707 / 95 CI: 1.861 / 7.384, 蠂 ~ (2 +) = 14.912 / P 0.05). Compared with C / T T / T genotype carriers, there were significant differences in calcium and phosphorus levels between the two groups (P 0.05), and there was no significant difference in serum magnesium and sodium levels between the two groups. There was no significant difference in low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) levels, but there was no significant difference in the levels of triacylglycerol (TGG), total cholesterol (TC) and high density lipoprotein cholesterol (HDL-C). There was no significant difference in genotype distribution between the other two groups, and there was no significant difference in electrolytes and lipids among the patients with different genotypes. Conclusion the polymorphism of SNP rs3752472 of Klotho gene is not different between the two groups. It is associated with the risk of calcium oxalate stone in Uygur population in Xinjiang. The rs3752472 polymorphism of Klotho gene in patients with higher levels of serum phosphorus and LDL-C may influence the formation of calcium oxalate stone by regulating the level of calcium phosphate metabolism and LDL-C.
【作者单位】：新疆医科大学第二附属医院泌尿外科;兰州大学第一医院泌尿外科;
【基金】：国家自然科学基金资助项目(编号:81460139)
【分类号】：R691.4

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