膀胱癌组织miR-451表达及其与E-cadherin和Vimentin表达相关性

发布时间：2018-02-24 01:51

本文关键词： 膀胱癌 miR- E-cadherin Vimentin　出处：《中华肿瘤防治杂志》2017年15期 　论文类型：期刊论文

【摘要】：目的侵袭与转移是影响膀胱癌患者疗效及导致死亡的主要因素,其具体机制尚不明确;研究表明miR-451与多种肿瘤的发生、发展密切相关。本研究分析miR-451在膀胱癌中的表达及其临床意义,观察miR-451表达改变对上皮型钙黏附蛋白(E-cadherin)和间质型骨架蛋白(Vimentin)表达的影响。方法收集2012-01-01-2015-12-31江西省人民医院收治的47例膀胱癌患者的癌组织及10例正常膀胱组织标本,运用实时荧光定量PCR(quantitative Real-time PCR,qPCR)检测miR-451的表达及运用免疫组织化学技术检测E-cadherin和Vimentin的表达,分析miR-451表达与膀胱癌临床病理因素,以及E-cadherin和Vimentin表达的关系。利用脂质体将miR-451拟似物(miR-451mimics)转染入膀胱癌5637细胞,采用蛋白质印迹法检测E-cadherin和Vimentin的表达。结果 miR-451在膀胱癌组织中的表达为0.14±0.017,明显低于正常膀胱组织(1.0),其表达和膀胱癌临床分期及病理分级密切相关,P0.01;E-cadherin在膀胱癌组织较正常组织中低表达,分别为36.17%和84.50%;Vimentin在膀胱癌组织及正常膀胱黏膜层中表达不明显。癌组织中miR-451表达与E-cadherin表达正相关,r=0.67,P=0.023;miR-451过表达后,E-cadherin蛋白表达上调(P0.001),Vimentin蛋白表达无明显改变,P0.05。结论MiR-451是膀胱癌潜在的调控子,可能是通过调控E-cadherin蛋白表达进而影响膀胱癌发生及进展。
[Abstract]:Objective invasion and metastasis are the main factors affecting the curative effect and death of bladder cancer patients, and the mechanism of invasion and metastasis is not clear. This study analyzed the expression of miR-451 in bladder cancer and its clinical significance. To observe the effect of miR-451 expression on the expression of E-cadherin (epithelial cadherin) and Vimentin (mesenchymal cytoskeleton). Methods 47 cases of bladder cancer and 10 cases of normal bladder tissues were collected from 47 patients with bladder cancer admitted to Jiangxi Provincial people's Hospital from January to January 2015-12-31. The expression of miR-451 and the expression of E-cadherin and Vimentin were detected by real-time fluorescence quantitative PCR(quantitative Real-time PCR and immunohistochemical technique. The expression of miR-451 and clinicopathological factors of bladder cancer were analyzed. And the relationship between E-cadherin and Vimentin expression. MiR-451 mimics were transfected into bladder cancer 5637 cells by liposome. Results the expression of E-cadherin and Vimentin in bladder cancer was 0.14 卤0.017, which was significantly lower than that in normal bladder tissue. The expression of E-cadherin was closely related to the clinical stage and pathological grade of bladder cancer. Weaving is lower in expression than in normal tissue. The expression of Vimentin was not obvious in bladder cancer and normal bladder mucosa. The expression of miR-451 and E-cadherin was positively correlated with the expression of E-cadherin in bladder carcinoma. Conclusion the expression of miR-451 and E-cadherin in bladder cancer tissues is not significantly changed after overexpression of P0.001Vimentin protein. Conclusion the expression of E-cadherin in bladder carcinoma tissues is positively correlated with the expression of E-cadherin protein. Conclusion the expression of Vimentin protein in bladder carcinoma tissues is positively correlated with the expression of E-cadherin protein. Conclusion the expression of Vimentin protein does not change significantly in bladder carcinoma tissues. The potential regulator of cancer, It may be that the expression of E-cadherin protein affects the development and progression of bladder cancer.
【作者单位】：江西省人民医院临床医学研究所;南昌大学研究生院医学部;江西省人民医院泌尿外科;
【基金】：江西省自然科学基金面上项目(20161BAB205278)
【分类号】：R737.14

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