基于诱导多潜能干细胞的Alport综合征蛋白质组学的生物信息

发布时间：2018-02-27 11:13

本文关键词： Alport综合征 iPSCs GO富集 KEGG通路　出处：《暨南大学学报(自然科学与医学版)》2015年02期 　论文类型：期刊论文

【摘要】：目的:探讨Alport综合征(AS)差异性表达蛋白质主要参与的GO富集分析和KEGG通路,为AS的进一步机制研究奠定基础.方法:10例AS患者与10例健康对照者(NC)作为实验对象.分别收集实验参与者尿液200 m L,并从尿液中分离尿肾脏管细胞,将尿肾脏管细胞诱导分化成多潜能干细胞(i PSCs),运用i TRAQ技术找出2组之间差异性表达蛋白质,对其进行生物信息学分析.结果:在2组样本中发现35种蛋白质具有差异性表达,包括18种上调与17种下调.差异性表达蛋白质GO富集主要聚集在细胞分子、细胞组成与细胞生物学过程.嘌呤代谢通路与丝裂原激活的蛋白激酶是主要的KEGG信号传导通路.结论:差异性表达蛋白质的生物信息学有助于AS发病机制研究,可作为机制研究参考和补充.
[Abstract]:Objective: to investigate the differential expression of protein in Alport syndrome, which is mainly involved in go enrichment analysis and KEGG pathway. Methods 10 patients with as and 10 healthy controls (n = 10) were selected as subjects. Urine samples of 200 mL urine were collected from participants, and renal catheterization cells were isolated from urine. Urinary renal tubular cells were induced to differentiate into multipotent stem cells. The differential expression proteins were identified by I TRAQ technique and analyzed by bioinformatics. Results: 35 proteins were found to be differentially expressed in two groups of samples. There are 18 upregulation and 17 down-regulation. Cell composition and cell biological process. Purine metabolic pathway and mitogen-activated protein kinase are the main KEGG signal transduction pathways. Conclusion: bioinformatics of differentially expressed proteins may contribute to the study of pathogenesis of as. It can be used as a reference and supplement for mechanism research.
【作者单位】：暨南大学附属第二医院深圳市人民医院临床医学研究中心;深圳市第三人民医院肾脏内科;广东医学院生物化学研究所;
【基金】：深圳市知识创新计划项目(JCYJ20140416122812045)
【分类号】：R692.3

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