微小RNA204在人肾透明细胞癌中的表达及临床意义

发布时间：2018-02-27 23:19

  本文关键词： 微小RNA 肾透明细胞癌 基因 Bcl-　出处：《重庆医学》2015年15期 　论文类型：期刊论文

【摘要】：目的研究微小RNA204(miR-204)在人肾透明细胞癌(RCCC)中的表达及临床意义。方法收集泌尿外科行手术切除的RCCC及对应癌旁正常肾脏组织标本共65例,运用qRT-PCR技术检测miR-204在RCCC及癌旁组织中的表达水平,统计分析miR-204表达水平与患者临床病理资料间的相关性;采用人工合成的miR-204模拟物转染人RCCCCaki-1细胞,分别采用qRT-PCR及Western-Blot检测转染后miR-204下游潜在靶点Bcl-2及SIRT1 mRNA及蛋白的表达变化。结果miR-204在RCCC组织中表达水平显著低于对应癌旁正常肾组织(P0.05);miR-204低表达与肿瘤体积增大(3cm,P0.05)、肿瘤淋巴结转移(P0.05)及高TNM分期(Ⅲ+Ⅳ期,P0.05)显著相关;miR-204转染人RCCCCaki-1细胞后可显著降低细胞内Bcl-2及SIRT1mRNA及蛋白的表达水平(P0.05)。结论 miR-204在RCCC组织中表达下调并与肿瘤恶性临床病理特征有关,miR-204可能通过下调Bcl-2及SIRT1的表达来抑制RCCC的发生、发展过程。
[Abstract]:Objective to study the expression and clinical significance of minimal RNA204miR-204 in human renal clear cell carcinoma (RCCC). Methods 65 cases of RCCC and adjacent normal renal tissues were collected from urological surgery. QRT-PCR technique was used to detect the expression of miR-204 in RCCC and adjacent tissues, and the correlation between miR-204 expression and clinical and pathological data of patients was analyzed statistically. Human RCCCCaki-1 cells were transfected with synthetic miR-204 mimics. QRT-PCR and Western-Blot were used to detect the expression of Bcl-2, SIRT1 mRNA and protein downstream of miR-204. Results the expression of miR-204 in RCCC tissues was significantly lower than that in normal renal tissues adjacent to cancer. The expression level of Bcl-2, SIRT1mRNA and protein in human RCCCCaki-1 cells was significantly decreased after transfection of p0.05) and high TNM stage (鈪，

本文编号：1544753