IL-6、TGF-β1在尿道损伤黏膜中的表达及作用

发布时间：2018-03-01 12:26

本文关键词： 尿道损伤 IL-6 TGF-β1 纤维化炎症　出处：《河北医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:探讨IL-6、TGF-β1在尿道损伤黏膜中的表达及作用。方法:1通过用导丝对小鼠尿道组织损伤建立尿道损伤模型。对正常尿道小鼠和尿道损伤小鼠的尿道组织进行苏木精-伊红染色(HE)观察和评价尿道黏膜的形态学改变。2实时定量PCR(q RT-PCR)检测正常尿道小鼠和尿道损伤小鼠的尿道组织IL-6、TGF-β1 mRNA的表达。3 IL-6、TGF-β1基因荧光探针联合MAC2原位杂交定位基因的表达。结果:1尿道损伤小鼠尿道组织增生明显,黏膜层厚度显著增加,管腔狭窄率增高。HE染色结果显示,与正常尿道组织相比,尿道损伤小鼠尿道组织增生显著,黏膜层厚度(303.2±21.4μm)显著增加(P0.05),尿道管腔狭窄率达90.67%,显著大于正常尿道组织的41.56%(P0.01)。2尿道损伤组织IL-6、TGF-β1 mRNA表达量显著高于正常尿道组织。实时定量PCR结果显示,正常尿道组织的IL-6、TGF-β1 mRNA表达增加仅为0.9和1.2倍,而尿道损伤组织表达增加各为2.5和4.3倍,表达显著增加。3尿道损伤组织中的炎症浸润增加,主要表现为炎性因子IL-6、TGF-β1表达增加及巨噬细胞浸润加剧。IL-6、TGF-β1基因荧光探针结合MAC2免疫染色的原位杂交结果显示,IL-6探针-MAC2、TGF-β1探针-MAC2阳性率在尿道损伤组织中显著高于正常尿道组织。结论:IL-6和TGF-β1在尿道损伤组织中高表达水平,促使炎性细胞的激活和浸润,两者相互作用,导致尿道黏膜组织慢性增生、纤维化,促进尿道狭窄的形成和发展。
[Abstract]:Objective: to investigate the expression and role of IL-6 TGF- 尾 1 in urethral mucous membrane injury. Methods: the urethral injury model of mice was established by using the guide wire. The urethral tissue of normal urethral mice and urethral injury mice were treated with hematoxylum thuringiensis. Observation and evaluation of morphologic changes of urethral mucosa by sperm-eosin staining. 2 Real-time quantitative PCR(q RT-PCR) to detect the expression of IL-6TGF- 尾 1 mRNA in urethral tissues of normal and injured urethra mice. The expression of IL-6 TGF- 尾 1 gene fluorescence probe combined with MAC2 in situ hybridization assay was used to detect the expression of IL-6 TGF- 尾 1 gene in urethral tissue of mice with urethral injury. Results the urethral tissue proliferation in mice with urethral injury was obvious. Compared with normal urethral tissue, urethral tissue proliferation in mice with urethral injury was significantly higher than that in normal urethral tissue. The mucosal thickness (303.2 卤21.4 渭 m) significantly increased the expression of IL-6 TGF- 尾 1 mRNA in urethral tissue, and the urethral stricture rate was 90.67, which was significantly higher than that in normal urethral tissue. The expression of IL-6 TGF- 尾 1 mRNA in urethral injury tissue was significantly higher than that in normal urethral tissue. The expression of IL-6 TGF- 尾 1 mRNA in normal urethra was only 0.9-fold and 1.2-fold higher than that in urethral injury, and the expression of IL-6TGF- 尾 _ 1 in urethral injury was increased by 2.5 and 4.3 times, respectively. The expression of IL-6 TGF- 尾 _ 1 increased significantly in urethral injury tissue. The results of in situ hybridization with fluorescence probe of TGF- 尾 1 gene and MAC2 immunostaining showed that the positive rate of IL-6 probe -MAC2TGF- 尾 1 probe in urethral injury tissue was significantly higher than that of normal tissue. Conclusions the expression of TGF- 尾 1 and IL-6 in urethral injury tissue is higher than that in urethral injury tissue. Promote inflammatory cell activation and infiltration, the interaction between the two leads to chronic hyperplasia of urethral mucosal tissue, fibrosis, promote the formation and development of urethral stricture.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R695

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