CUEDC2在糖尿病肾病小鼠肾脏中表达及其与24h尿白蛋白、血肌酐的相关性

发布时间：2018-03-02 01:17

本文关键词： 糖尿病肾病 CUEDC db/db小鼠尿白蛋白血肌酐　出处：《实用医学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的:研究CUEDC2在糖尿病肾病小鼠肾脏中的表达、定位及与肾功能相关性。方法:选用db/db小鼠为实验组,db/m小鼠为对照组,相同饲养条件下,24周时取组织样本;采用PAS,HE及Masson染色观察肾脏病理改变;Western Blot,免疫组织化学法,Real time PCR检测肾组织CUEDC2的表达、定位;由本院检验科测定血肌酐、24 h尿白蛋白。结果:CUEDC2蛋白表达定位于肾小管上皮细胞中。24周时,db/db小鼠出现肾小球硬化,肾间质增宽、炎症细胞浸润;较同期db/m小鼠,系膜基质明显扩张,肾小球及肾小管间质胶原染色增多,肾组织CUEDC2蛋白及mRNA均下调(P0.05)。db/db小鼠肾脏中CUEDC2含量分别与24 h尿白蛋白与血肌酐呈明显负相关(P0.05)。结论:CUEDC2在糖尿病小鼠肾脏中下调,并与尿白蛋白、血肌酐含量呈负相关。
[Abstract]:Objective: to study the expression and localization of CUEDC2 in the kidney of diabetic nephropathy mice and its correlation with renal function. Methods: the db/db mice were selected as the experimental group as the control group, and the tissue samples were taken at 24 weeks under the same feeding conditions. The pathological changes of kidney were observed by PAS-HE and Masson staining. Real time PCR was used to detect the expression and localization of CUEDC2 in renal tissue. The 24 h urinary albumin of serum creatinine was measured by our laboratory. Results the expression of the protein was localized in the renal tubular epithelial cells. At 24 weeks, glomerulosclerosis, renal interstitial widening and inflammatory cell infiltration were observed in the mouse, compared with the db/m mice in the same period. Mesangial matrix dilated obviously, glomerular and renal tubulointerstitial collagen staining increased, Both CUEDC2 protein and mRNA in renal tissue were significantly negatively correlated with 24 h urinary albumin and serum creatinine in P0.05 路dbdb mice. Conclusion\\% CUEDC2 is down-regulated in diabetic kidney, and negatively correlated with urinary albumin and creatinine content.
【作者单位】：南方医科大学;广东省医学情报研究所综合评审部;广州军区总医院肾内科;
【基金】：国家自然基金资助(编号:81270895)
【分类号】：R587.2;R692.9

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